粘质沙雷氏菌摇瓶发酵产灵菌红素的工艺优化-食品科学.pdfVIP

※生物工程 食品科学 2015, Vol.36, No.13 119 粘质沙雷氏菌摇瓶发酵产灵菌红素的工艺优化 张丹峰，杨培周，操丽丽，姜绍通* （合肥工业大学生物与食品工程学院，安徽省农产品精深加工重点实验室，安徽合肥 230009 ） 摘 要：为提高灵菌红素产率，本研究通过单因素试验和正交试验优化粘质沙雷氏菌发酵培养基组分和发酵条件， 结果表明，最佳培养基配方为：一级大豆油、蛋白胨、NaCl和K HPO 的添加量分别为4 mL/100 g 、1.5%、0.15%和 2 4 0.15%；最佳发酵条件为：温度30 ℃、接种量1%、装液量70 mL/250 mL三角瓶、振荡培养转速200 r/min，发酵36 h 后，灵菌红素的产量最大，为2.98 g/L 。粘质沙雷氏菌的胞外脂肪酶活力为15 U/mL。 关键词：粘质沙雷氏菌；灵菌红素；单因素试验；正交试验 Optimization of Shake Flask Cultivation Conditions for Prodigiosin Production by Serratia marcescen * ZHANG Danfeng, YANG Peizhou, CAO Lili, JIANG Shaotong (Key Laboratory for Agricultural Processing of Anhui Province, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China) Abstract: This paper reports the optimization of medium components and fermentation conditions for improved production of prodigiosin by Serratia marcescen using single factor and orthogonal array designs. Results indicated that the optimal medium consisted of 4 mL/100 g of soybean oil, 1.5% of peptone, 0.15% of sodium chloride and 0.15% of potassium dihydrogen phosphate, and the optimal fermentation conditions were determined as 30 ℃, an inoculum size of 1%, 70 mL of medium contained in a 250-mL flask, and cultivation with shaking at 200 r/min. The maximum prodigiosin production achieved after 36 h of cultivation under the optimized conditions was 2.98 g/L, and the activity of extracellular lipase from S. marcescens was 15 U/mL. Key words: Serratia marcescens; prodigiosin single factor method; orthogonal array design ; 中图分类号：Q939.9