热毒宁注射液对IL-1、IL-6、ET-1、PGE2炎性介质的影响.doc

热毒宁注射液对IL-1、IL-6、ET-1、PGE2 王开富1#，萧伟2、3△ 王振中2、3，徐丽君1，毕宇安2、3， , 孙兰2、3，邹欣1，龚菂1，黄光英,1△ 1华中科技大学同济医学院附属同济医院，武汉，430030 2江苏康缘药业股份有限公司，江苏 连云港 222001 3中药制药过程新技术国家重点实验室, 江苏 连云港 222001 摘要：目的：探讨热毒宁注射液抗炎、清热解毒的作用及其机制，方法：分别以二甲苯致小鼠耳肿、角叉菜胶致大鼠足趾肿胀、冰醋酸致小鼠毛细血管通透性增加等炎症模型；以内毒素感染家兔致内毒素性发热模型，观测小鼠耳肿胀及大鼠足趾关节肿胀度，检测IL-1、IL-6、ET-1）、PGE2) 等炎症介质，结果：热毒宁注射液10.15g/kg、5.08g/kg2.54g/kg剂量组在2小时方显效，抑制作用可持续至4小时；10.15g/kg剂量组对二甲苯致小鼠耳肿胀有明显的抑制作用，并能降低小鼠毛细血管通透性；注射内毒素2h，模型组家兔肛温值居高不下，但热毒宁2.54 g/kg、5.08g/kgIL-1、IL-6、ET-1、PGE2；热毒宁注射液对对IL-1、IL-6热毒宁注射液的IL-1、ET-1、IL-6、PGE2E-mail:wkf58@163.com 共同通讯作者一：萧伟，男，研究员，高级工程师，E-mail：wzhzh-nj@ E-mail: gyhuang@；2010CB735604 The influence of Reduning injection on the inflammatory mediums of IL-1, IL-6, ET-1 and PGE2 * Wang KaiFu 1 #, XiaoWei 2, 3△, WangZhenZhong 2, 3, XuLiJun 1, BiYuAn 2, 3, SunLan 2, 3, zou xin 1, Gong di 1, Huang GuangYing 1△ 1Tongji medical college affiliated tongji hospital, huazhong university of science and technology, wuhan, 430030 2 Jiangsu kang Yuan pharmaceutical co., LTD., lianyungang jiangsu, 222001 3National key laboratory for advanced technology in pharmaceutical process of Traditional Chinese medicine, lianyungang, jiangsu, 222001 Abstract: objective: to study the effect of Reduning injection in anti-inflammation and clearing heat and detoxicating and its mechanism. methods: Inflammation models were established by xylene-induced mouse ear swelling, carrageenin-induced rat toe swelling and glacial acetic acid-induced increase of blood capillary permeability in mice, respectively. Endotoxin heating model was induced by infecting the rabbit with toxin. The swelling degree of mice ear and rat digits joint was observed. The inflammatory mediums of IL-1, IL-6, endothelin (ET-1), prostaglandin E2 (PGE2) were detected. Results: Reduning injection with doses of both 10.15 g/kg and 5.08 g/kg inhibited the rat toe swelling caused by carrageenin, with the effect appearing 1 hour and continuing up to 4 hours after receiving the injec