ITS序列鉴别三七及其伪品.pdfVIP

第30 卷 第2 期 商洛学院学报 Vol．30 No．2 2016 年4 月 Journal of Shangluo University Apr. 2016 doi:10.13440/j.slxy.1674-0033.2016.02.014 ITS 序列鉴别三七及其伪品 赵月梅， 韩冰 （商洛学院生物医药与食品工程学院，陕西商洛 726000） 要 列作为 其伪品的基源植物。 通过 接测 摘 用 序 条形码来区分三七及 直 : ITS DNA PCR 序和在 中进行序列下载两种方法共选用了 条三七正品及伪品的 序列做变异 genbank 16 ITS 分析， 经ClustalX 2.0 软件比对后用MEGA4.0 计算种间的K2P 距离并基于该距离建立了NJ 系统树，并计算了“barcode gap”。 结果显示，三七与其伪品水田七、姜黄、竹节参、 白术、藤三七 可明显区分开。 表明ITS 序列可作为区分三七和其常见伪品的参考DNA 条形码。 关键词： ；三七；伪品； 条形码 ITS DNA 中图分类号： 文献标识码： 文章编号： （ ） R282.7 A 1674-0033 2016 02-0061-04 On Identification of Panax Notoginseng and its Adulterants Using ITS ZHAO Yue-mei, HAN Bing （College of Biopharmaceutical and Food Engineering, Shangluo University, Shangluo 726000, Shaanxi） Abstract:the ITS sequence is used as the DNA barcode to distinguish the Panax Nnotoginseng and its adulterants in the market. 16 ITS sequences. which were obtained from both PCR sequencing and downloading from genbank were compared by ClustalX 2.0 software, then MEGA4.0 was used to calculate the K2P distance and produce the NJ phylogenetic tree. The results showed that Panax notoginseng and its adulterants Curcuma longa, Atractylodes macrocephala, Tacca plantaginea, Anredera cordifolia, Panax japonicas can be clearly separated. the ITS sequence can be used as a reference DNA bar