(精选)MiRNA的鉴定与分析(identification and analysis of conserved and novel microRNAs)教学课件.pptVIP

Acknowledgements SONNANTE Gabriella PIGNONE Domenico CATALANO Domenico FRACCHIOLLA Antonio CATTONARO Federica FELICE Nicoletta Istituto di Genetica Vegetale Institute of Plant Genetics Thank you Eamens A. et.al. Plant Physiol. 2010:147:456-468 The two-nucleotides at 3’ ends of the liberated miRNA duplex are methylated by the sRNA-specific methyltransferase HUA ENHANCER1 (HEN1) The guide miRNA strand is then incorporated into AGO proteins, the catalytic center of RNA-induced silencing complex (RISC), to carry out the silencing reactions Interaction with target mRNA leads to degradation or translational inhibition * * * * * * * * * * * * * * * * * * * * * * * * IDENTIFICATION AND ANALYSIS OF CONSERVED AND NOVEL microRNAs FROM ARTICHOKE BY DEEP SEQUENCING Domenico DE PAOLA, Domenico CATALANO, Nicoletta FELICE, Federica CATTONARO, Domenico PIGNONE Gabriella SONNANTE Istituto di Genetica Vegetale Institute of Plant Genetics Artichoke (Cynara cardunculus var. scolymus) Asteraceae family (lettuce, sunflower) The nutritional benefits of artichokes include high levels of potassium, folate, magnesium, vitamin C. Rich in antioxidant compounds, mainly phenylpropanoids (e.g. chlorogenic acid, caffeic acid, dicaffeoylquinic acids, flavonoids) Globe artichoke is widely cultivated in the Mediterranean basin and is an important market crop in this region. Used not only as a food, but also for extraction of pharmaceutical compounds World production 1.200.000 t Italy 470.000 t (ca. 40%) Apulia 156.000 t Salinity is becoming an increasingly global problem and affects approximately 20% of global irrigated agricultural land Artichoke is considered a crop moderately tolerant to salt Plants cope with salt stress by altering the expression of large sets of genes involved in a variety of biochemical and physiological processes (e.g. energy metabolism, signal transduction, transcription, protein biosynthesis and decay, photosynthesis…) Post-transcriptional re