(精选)SiRNA and MiRNA教学课件.pptVIP

Gene;Gene;Gene;Gene;Gene;Micro RNA (miRNA) lin-4 Lee et al., Winghtman et al., 1993 Correct timing of development in C. elegans, two miRNA, can fold in hairpin structure Let 7 Reinhart et al., 2000 (Ruvkun G. Lab) Additional small RNA coding miRNA involved in control of developmental timing ;siRISC;Nat Rev Mol Cell Biol. 2007 Jan;8(1):23 ?????????????????? ;Comput Biol Chem. 2006 Dec;30(6):395-407; The seven components of the MiRscan score for mir-232 of C.?elegans/C. briggsae. In parenthesis are the scores for each component, which were added together to give the total score of 13.9.? MiRscan predictions are visualized within the consensus C.?elegans/C. briggsae secondary structure, as generated by using ClustalW (Thompson et al. 1994) and Alidot (Hofacker and Stadler 1999). ;The distribution of MiRscan scores for 35,697?C.?elegans sequences that potentially form stem loops and have loose conservation in C.?briggsae ;Computational identification of Drosophila microRNA genes ;mFOLD 3.1;Preferential divergence of miRNAs within their loop sequences is illustrated by let-7. The Drosophila orthologs of let-7 contain three mismatches and one gap within the loop, whereas both arms have been completely conserved. ;Distribution of the top 2,996 candidates binned by helical/free energy score (white bars), of which 570 passed subsequent conservation filters (green bars). 21/24 members of the reference set received a score of 16 or higher, and 20 of these passed the conservation filters. List of the top 124 miRseeker candidates; members of the reference set are highlighted in green, newly identified miRNAs from this study in blue, and additional third-species-conserved candidates in orange. The vast majority of the highest scoring candidates are bona fide. ;Results;Pipeline structure, use cut-offs and filtering/eliminating sequences as pipeline proceeds. Sequence alignment alone used to infe