灯盏花素注射液对家兔缺血再灌注损伤肠系膜微循环的影响.docVIP

灯盏花素注射液对家兔缺血再灌注损伤肠系膜微循环的影响 ? 576?新乡医学院JournalofXinxiangMedicalCollegeVo1.24No.6Nov.2007 灯盏花素注射液对家兔缺血再灌注损伤肠系膜微循环的影响 刘东华,张新华,刘晓丽 (1.新乡医学院生命科学技术系,河南新乡453003;2.周13职业技术学院,河南3.新乡医学院机能 学实验室,河南新乡453003) 摘要:目的探讨灯盏花素注射液对家兔缺血再灌注损伤肠系膜微循环的保护作用.方法将15只家兔随机 分为模型组,预防组,治疗组,在BI2000医学图像分析系统下观察并记录缺血30min和再灌注30min时家兔肠系膜微 动脉管径(DA),微静脉管径(DV),血液流态(BFS),微血管数目(CV)和红细胞聚集(EA).结果缺血30min时,各 组与缺血前比较,预防组BFS权分值增加(Plt;0.05),其他4项指标变化不显着;模型组,治疗组DA,DV缩小(Plt; 0.05),BFS权分值增加(Plt;0.001),CV减少,EA明显(Plt;0.O1);与模型组比较,预防组DA舒张,EA明显(Plt; 0.05),BFS权分值减小,CV增多(Plt;0.O1),治疗组微循环变化不显着;预防组与治疗组比较DA舒张,CV增多(Plt; 0.05),BFS权分值减小,EA明显(Plt;0.O1).再灌注30min时各组与其缺血前比较,模型组DA,DV缩小,CV减少,EA 明显(Plt;0.O1),BFS权分值增加(Plt;0.001),预防组CV增多,BFS权分值减小(Plt;0.05),治疗组微循环变化不显着; 与模型组比较,预防组DA,DV舒张,BFS权分值减小,CV增多,EA明显(Plt;0.O1),治疗组DA,DV舒张(Plt;0.05), BFS权分值减小,CV增多,EA明显(Plt;0.O1);预防组与治疗组比较,DV舒张(Plt;0.05),BFS权分值减小(Plt;0.O1). 结论灯盏花素注射液对家兔缺血再灌注时肠系膜微循环损伤有一定的保护作用. 关键词:缺血一再灌注损伤;微循环;灯盏花素注射液 中图分类号:R972文献标识码:A文章编号:1004-7239(2007)06—0576一o4 Theprotectiveeffectofbreviscapineinjectiononmicrocirculationofmesenteryinrabbitswithische— mia—relmrtUsioninjury LIUDong.hua,ZHANGXin.hua.LIUXiao.1i (1.LifeScienceandTechniqueDepartmentofXinxiangMedicalCollege,Henan453003,China;2.ZhoukouVocationalTechnology College,Henan466001,China;3.FunctionalScienceLaboratoryofXinxiangMedicalCollege,ltenan453003,China) Abstract:ObjectiveTostudytheprotectiveeffectofbreviscapineinjectiononmicrocirculationofischemia—reperfusion (IR)injuryinmesenteryofrabbit.MethodsFifteenrabbitswererandomlydividedintomodelgroup,preventiongroupand treatmentgroup.Thediametersofmicroarteriole(DA),Thediametersofmicrovenule(DV),thebloodflowshape(BFS),the numberofcapillaryvessel(CV)anderythrocyteaggregation(EA)werestudiedbyBI-2000imageanalyzeratthepointof30 minofischemiaand30minofreperfusionrespectively.ResultsAtthepointof30minofischemia,comparedwithpriorische— mia,theDA,DVandCVdecreased,weighedvalueofBFSandEAincreasedremarkedlyinmodelgroupandtreatmentgroup(P lt;0.O1).Preventiongroupshowednosignificantdiffere