8周有氧运动调节appps1小鼠皮层rho gtpasescofilin信号通路 word格式.docxVIP

was significantly decreased in AE(P0.05), and the expression of cofilin was significantly decreased in prefrontal cortex(P0.01), and the level of Syn was increased. At the same time, the cellular structure disorder changes were ameliorated significantly. 5) The expression of RhoA was increased, while Rac1 and Cdc42 wasdecreased significantly(P＜0.05)in prefrontal cortex in 6-month-old APP/PS1 mice.After 8w aerobic exercise, the changes were ameliorated significantly. The activity of Cdc42 in prefrontal cortex was decreased in AS,while after 8w aerobic exercise Cdc42’s activity was increased significantly(P＜0.05).Conclusion: Aerobic exercise may reduce the extent of Aβ deposition in cortex of 6-month-old APP/PS1 transgenic mice, thus resulting in inhibiting cofilin aggregation and synaptic loss, which suggest, to an extent aerobic exercise would reduce neuropathology, rescue brain structure under neuropathological conditions by up-regulating the activity of Cdc42. That may be an important molecular mechanismof exercise-induced learning and memory ability promoting．Key Words: aerobic exercise; Rho GTPases/cofilin; Aβ; APP/PS1 transgenic mouse论文随机验证编号：BT123054935470VI目录1 前言 11.1 选题依据 11.2 研究目的 21.3 技术路线图 22 文献综述 32.1 突触丢失与 AD32.1.1 突触丢失的形式与发生机制 32.1.2 突触标记物 52.2 Aβ 沉积与突触丢失72.2.1 不同构象 Aβ 沉积的作用机制72.2.2 Aβ 的氨基酸序列和翻译修饰92.2.3 基于 APP 的转基因动物模型9cofilin 聚集与突触丢失 10cofilin 上游 Rho GTPases 家族 11RhoA 13Rac1 14Cdc42 152.5 运动与 AD 162.6 小结 183 研究对象与研究方法 193.1 研究对象 193.2 主要仪器 193.3 主要试剂及耗材 20论文随机验证编号：BT123054935470VII3.4 常用试剂的配制 213.5 运动方案 223.6 行为学测试 223.6.1 跳台回避实验 223.6.2 旋转杆平衡实验 233.7 血清皮质酮的测定 243.8 免疫荧光染色 243.9 免疫组化检测 253.10 Western Blot 253.11 透射电镜 263.12 Pull Down Assay 263.13 数据处理与分析 274 实验结果 284.1 行为学测试结果 284.1.1 跳台回避实验 284.1.2 旋转杆平衡实验 284.2 有氧运动对血清皮质酮水平的影响 294.3 有氧运动对额叶、颞叶 Aβ 含量的影响 304.4 有氧运动对额叶、颞叶 cofilin 含量的影响 324.5 有氧运动对额叶、颞叶 Syn 的影响 334.6 有氧运动对额叶、颞叶 Rho GTPases 表达和活性的影响 364.6.1 有氧运动对 RhoA 总蛋白的表达与 RhoA-GTP 活性的影响 364.6.2 有氧运动对 Rac1 总蛋白的表达与 Rac1-G