内蒙古地区骆驼生活环境蝇种分类与coi基因序列比较-预防兽医学专业论文 word格式.docxVIP

Classification ofFlies Species around Carnels Living Environment in Inner Mongolia and COI Gene ComparisionAbstractTo study the possibi lity of some kinds of tlies served as the vectors of Parabron ema skrjabini，the tlies species and thei r distribution in camels living environment were investigated in the prairie. Also，we tried to find out the relationship among the flies bymolecular biological method. Firstly，the flies were collected from three areas of deserted grasslands between July and September from 2007 to 2009 in lnner Mongolian RegionThen，they were identified and classified by conventional morphological methodMeanwhile，genome DNA of the flies was exstracted and COI genes were amplified with w1iversal primers (UEA3 and UEA 10) of files. Later，cloning and sequencin g of the PCR products were done. The COI sequence was analysed by software DNASTAR，thenphylogenetic tree and homology were also established .The result showed that the 25 investigaed flies species belonged to 17 genera of 7 臼milies and another 2 families were not identifi ed in genera level. Among aLl of them，12 species were firstly reported in Inner Mongolia Region. The prevalent flies were Hydrophoria ambigua up to 37.2%，followed by Haematobia titillans (23.0%) inBayannaoer district. ln Alasan district，the highest percent of tlies belonged to Coelopidae (about 31.5%)，thenNorellia sl!切lata (26.5%)，and other species were a few. But，the dominant tlies species belonged to Trixoscelidae (around 43.4%) from Wulanchabu district，followed by Lispe litorea and L. consanguinea (about 10.6% and 12.4%).Seen tì-om the result of molecular taxonomy ，the variability of nineteen C01 sequenceswas from 0.1% to 22.8% and homology 飞Nas between 78.2% and 99.9%. The differences in most of flies from various families and various genera of the same family were significant. ln the nineteen C01 sequences，containment of A+T was from 67.44% to 72.62% and it wasfrom 27.38% to 3