dc分型方法及迁移方式分子基础的发展word格式论文.docxVIP

英文摘要Purpose:Toinvestigate the effectsof labelingHuman hepG2 cellswithdifferentconcentrat ionsofSPIO,and to investigate the feasibilityof in vitroMR imaging. Methods:1.Choo se the suitable concentrationsofSPIO tolabel Human hepG2 cells. Labeling the Human hepG2cellswith SPIO bythe concentrationsof0ug/ml,125ug/ml,250ug/ml,375ug/ml,500ug/ ml,625ug/ml,750ug/ml,,875ug/mlrespectively. Prussian blue stain and Transmission Electron Microscope(TEM) were performed forshowingintracellulariron. Then throughMTTcel lproliferation cytotoxicity detection kitto Measure the cytoactive ofHuman hepG2 cellst hatwere labeled bydifferentconcentrationsofSPIO.2.ChoosethebestconcentrationofSP IO to label HumanhepG2 cellsbyin vitro MR imaging. Imaging different densityofHuman hepG2 cells(1×102,1×103,1×104,1×105,1×106,1×107) that waslabeled bydifferentconcentrationsofSPIO(0ug/ml,375ug/ml,500ug/ml).The signal intensityofcells were evaluated by3.0 MRIwith T2WIsequencesinvitro. Results:Prussianblue stain showthatbythe increasing ofthe SPIO thebluenessbecome darker. Whenthe concentration ofSPIO was 0ug/ml,the labeling rate was less than 5%.While the labeling rate was more than 90% w hen the concentrationsofSPIO were 125ug/mlto 875ug/ml(P0.05). MTTtestshowthatt he cytotoxicityofSPIO is comparatively large when the concentration islargerthan 625u g/ml(P0.05). There wasnot nolyinverselycorrelation between the concentration ofSPIOand the signal intensity（P0.05）,butalsothe labelingcellpopulationand the signalintensity(P0.05). Conclusions: Human hepG2cellscould be labeled effectively with SPIO, and MRIcould beused to monitorthese labeled cellsinvitro.Key word：superparamagnetic iron oxide（spio）；Human hepG2 cells ;in vitro imaging ;MRI前言肝细胞癌(hepatocellularcarcinoma, HCC)是肝脏常见的原发性肿瘤，其发病机制复杂、侵袭性强转移率高的诸多特点致使治疗方法有限、生存率极低，已经严重威胁全球人类的生命健康，并为全球性问题，每年发病人数56万，导致55万人死亡。我国肝癌高发情况十分严重，全球每年新诊断的肝癌患者有近一半在中国，且发病率仍在不断上升，死亡人数占全世界肝癌的45%。据广东省卫生厅疾病控制处资料显示，我省