dna电化学生物传感器的构建及抗氧化活性分析word格式论文.docxVIP

on the CS/ds-DNA/Ag-PGL/GCE. At first, the Ag+ and L-glutamic acid (PGL) monomer was co-polymerized on a GCE by repetitive cyclic voltammetry. Then, a layer of CS/ds-DNA was covered on the outside of the membrane of Ag-PGL by casting method. The modified electrode was put into the Fenton solution (Fe2+/H2O2). Fe2+ reacted with H2O2 and generated ·OH. The ·OH attacked the topside of the ds-DNA and induced ds-DNA damage. The ds-DNA damage and the antioxidant capacities of the orange juice beverage and AA were monitored by linear sweep voltammetry (LSV) and EIS. The experimental conditions, such as the ratio of Fe2+ and H2O2, the pH value of the Fenton solution, the treatment of time were optimized. UV-vis was also used to detect the ds-DNA damage and antioxidant capacity, and almost the same conclusions as electrochemical method were obtained.Keywords: DNA; Biosensor; Electrochemistry; Antioxidant activity; Application目 录第一章 绪论 ............................................................................................................................... 1一 DNA 电化学生物传感器11 DNA 杂交电化学生物传感器12 DNA 损伤电化学生物传感器1二 抗氧剂 ................................................................................................................................... 31 抗氧剂的定义及分类 ......................................................................................................... 32 抗氧剂的抗氧化机理 ......................................................................................................... 33 抗氧剂电化学生物传感器 ................................................................................................. 44 抗氧化性研究的其他方法 .................................................................................................. 5三 论文工作的主要内容 ........................................................................................................... 5 第二章 DNA 在 CS/ds-DNA/Hb/MWCNTs 膜内的电化学行为 .............................. 6 一 引言 ..............................................................................