egfr jnk信号通路在系膜细胞增殖中的作用及肝素对其影响word格式论文.docxVIP

AbstractObjectiveHeparinisawellproliferationinhibitorofglomerularmesangialcells(GMCs)bothinanimalmodelsandinvitro,buttheexactmechanismhasstillnotbeenexplained.TheaimsofthisstudyweretoinvestigatewhethertheEGFR-JNK SignalingPathwayplay animportantroleinratsGMCsProliferationandtheeffect of heparinInIt.MethodsFourgroupswereestablished:GMCsgroup,LPSgroup(GMCs+LPS), heparingroups(GMCs+LPS+heparin),AG1478group(GMCs+LPS+AG1478). GMCs proliferationweredetectedbyMTTat24h afterbeculture. Theexpressionsof EGFRandP-JNKwereassayedbywesternblotandimmunohistochemisty.Results(1)Intheheparingroupwiththeconcentrationof250ug/mlandtheAG1478groupoftheconcentrationof20umol/L,theproliferationofGMCswerelowerthanintheLPSgroupand100ug/ml,500ug/mlheparingroups(p0.01).(2) On normalculturecondition,GMCscanexpressacertainamountofEGFRandP-JNK, theexpressionisup-regulatedinLPSgroup(p0.01),theexpressionofEGFRandP-JNKinheparingroupswerelowerthaninLPSgroup(p0.05),buttherewasno statisticaldifferencewhencomparedwiththeGMCsgroup.(3)Theexpressionof P-JNKinAG1478groupwaslowerthanthatinLPSgroup(p0.05),anddidnotdifferfromthatinheparingroupsandGMCsgroups.ConclusionThemoderateconcentrationofheparinandAG1478canobviouslyinhibittheproliferationofGMCswhichinducedbyLPS,theheparincaninhibittheproliferationofGMCsbydown-regulatetheEGFR-P-JNKsignalingpathway.Keywords:GMCsproliferation，EGFR，P-JNK，AG1478中英文缩略词表英文缩写中文解释GMCs大鼠肾小球系膜细胞LPS脂多糖EGFR表皮生长因子受体JNKc-Jun N端激酶P-JNK磷酸化JNKWB免疫印迹法MTT甲基噻唑基四唑PAGE聚丙烯酰胺凝胶电泳DMSO二甲基亚砜PDGF血小板源性生长因子TNF-α肿瘤坏死因子-αECM肾小球系膜外基质HB-EGF肝素结合样表皮生长因子VEGF血管内皮生长因子ERK1/2细胞外信号通路1/2PLC磷脂肌醇特异性磷脂酶CMAPK促分裂素原活化的蛋白激酶NF-KB核因子-kBPKC/PKA蛋白激酶C/ACaMK-Ⅱ钙调蛋白激酶ⅡROS活性氧自由基HSPG人硫酸肝素糖蛋白PI3K磷脂酰肌醇3-激酶STAT信号传导及转录激活因子VSMC血管平滑肌细胞cAMP腺苷-3,5-环化一磷酸AKT又称PKB，蛋白激酶BAP显色法碱性磷酸酶显色体DAB3,3-二氨基联苯胺AP-1转录因子复合物-1Ras/Raf为一种致癌基因独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版