基于保肝活性的禹州漏芦品质评价分析-evaluation and analysis of quality of luzhou in yuzhou based on hepatoprotective activity.docxVIP
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基于保肝活性的禹州漏芦品质评价分析-evaluation and analysis of quality of luzhou in yuzhou based on hepatoprotective activity
目 录摘 要 ................................................................. 1Abstract3前 言 ................................................................. 51研究背景 ........................................................... 52中药谱效关系 ....................................................... 63研究内容 ........................................................... 9第一部分 禹州漏芦醇提物对四氯化碳所致小鼠急性肝损伤的影响 ........... 11 1材 料 ............................................................. 111.1试验动物 .................................................... 111.2药品与试剂 .................................................. 111.3仪 器 ....................................................... 121.4样品的制备 .................................................. 122方 法 ............................................................. 122.1CCl4 致小鼠急性肝损伤模型的建立122.2实验方法 .................................................... 122.3生化指标的检测 .............................................. 132.4组织病理学观察 .............................................. 132.5统计学方法 .................................................. 133结 果 ............................................................. 133.1禹州漏芦醇提取对小鼠一般活动状况的影响 ...................... 133.2禹州漏芦醇提取物对 CCl4 致小鼠急性肝损伤模型血清中 ALT,AST 的影响................................................................. 143.3禹州漏芦醇提取物对 CCl4 致小鼠急性肝损伤模型肝组织中 SOD, GSH-Px, MDA 的影响153.4禹州漏芦醇提物对 CCl4 致急性肝损伤模型小鼠的肝组织病理形态学的影 响16第二部分禹州漏芦保肝活性部位的研究 ................................ 19 1材料 .............................................................. 191.1试验药物 .................................................... 191.2试验动物 .................................................... 191.3试 剂 ....................................................... 191.4仪 器 ....................................................... 202方 法 ......................................................... 202.1生化指标的检测 .............................................. 202.2组织切片观察 ................................................ 203结 果 .................................................
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