Soluble endoglin和sFlt1在子痫前期患者血清中的表达.docVIP

Soluble endoglin和sFlt1在子痫前期患者血清中的表达 作者：邢静,陈丽君 单位：山东大学齐鲁医院妇产科， 济南 【摘要】目的 探讨可溶性CD105淋巴细胞抗原及可溶性血管内皮生长因子受体1(sFlt1)在子痫前期患者血清中表达水平的变化及意义。方法 采用双抗体夹心酶联免疫吸附(ELISA)法测定子痫前期组、子痫前期各个亚组及正常晚期妊娠组血清sEng及sFlt1的水平。结果 ①子痫前期组孕妇血清sEng、sFlt1水平分别为(5.27plusmn;1.05)ng／mL、(391.14plusmn;70.25)pg／mL，正常晚期妊娠组分别为(1.26plusmn;0.40)ng／mL、(27.57plusmn;4.36)pg／mL，两组比较差异有统计学意义(P＜0.01)；②轻度子痫前期组sEng水平与重度子痫前期组比较差异有统计学意义(P＜0.01)，两组sFlt1水平比较差异无统计学意义(P＞0.05)；③早发型重度子痫前期组sEng水平与晚发型重度子痫前期组比较差异有统计学意义(P＜0.01),两组sFlt1水平比较差异无统计学意义(P＞0.05)。结论 子痫前期患者血清中sEng、sFlt1水平显著升高，sEng在早发型重度子痫前期血清中升高更显著，对早期诊断子痫前期有重要意义。 【关键词】 子痫 可溶性CD105淋巴细胞抗原 可溶性血管内皮生长因子受体1 血管生成因子 　　blood of preeclampsia 　　XING Jing, CHEN Lijun 　　(Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University， Jinan 250012, China) 　　To investigate the expression and significance of serum soluble endoglin(sEng) and soluble fmslike tyrosine kinase 1 (sFlt1) in preeclampsia. Methods Serum levels of sEng 1 and sFlt1 were determined by enzyme linked immunosorbent assay. Results The serum level of sEng of the preeclampsia group (5.27plusmn;1.05)ng／mL was significantly higher than that of the controls ［(1.26plusmn;0.40)ng／mL，P＜0.01］, especially of earlyonset severe preeclampsia. The serum level of sFlt1 of the preeclampsia group (391.14plusmn;70.25)pg／mL was significantly higher than that of the third trimester group (27.57plusmn;4.36)pg／mL, but sFlt1 did not correlate with the most severe forms of the disease. Conclusion Serum levels of sEng and sFlt1 are significantly increased in preeclampsia, and the serum level of sEng is even higher in severe preeclampsia patients. 　　Key words： Preeclampsia； Soluble endoglin； Soluble fmslike tyrosine kinase receptor 1； Angiogenic factor 　　子痫前期是一种常见且严重影响母儿健康的妊娠期特有疾病。研究表明，子痫前期的发病起源于胎盘的病理生理改变，子宫螺旋动脉重铸障碍是子痫前期发病的中心环节，胎盘分泌的血管生成因子是影响子宫螺旋动脉重铸的重要因素［1］。可溶性CD105淋巴细胞抗原(sEng)是一种可溶性的转化生长因子(TGF)beta;亚型受体；可溶性血管内皮生长因子受体1(sFlt1)是一种有效的血管内皮生长因子(VEGF)和胎盘生长因子(PLGF)的抑制因子，二者均能减少血管发生［2］。为探讨子痫前期患者外周血中及其在子痫前期病理生理过程中的