季铵盐与制膜溶剂对电形成法制备囊泡影响-effect of quaternary ammonium salt and film-making solvent on preparation of vesicles by electro-forming method.docx
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季铵盐与制膜溶剂对电形成法制备囊泡影响-effect of quaternary ammonium salt and film-making solvent on preparation of vesicles by electro-forming method
the formation of GUVs. Then the optimum coating solvent which is also economic and environmentally friendly for the electroformation of vesicles is obtained.It shows that the optimal coating solvent is ethanol-water mixed solution, and the best volume ratio of water to ethanol are both 1:1 and 1:2. The optimal electric field condition is 5 V, 20 Hz. The operation process using ethanol is simple and convenient. Vesicles made by this method can exist a longer time, meanwhile, it removed the use of some toxic solvent such as chloroform and methyl in the experiment process, so this method is conducive to expand the application range of the vesicles.KEY WORDS: vesicles, phospholipids, carbon fiber, quaternary ammonium salts, electroformation, solvent目录目录 ..........................................................................................................................1第一章 前言...................................................................................................................11.1 囊泡..................................................................................................................11.1.1 囊泡概述 ...............................................................................................11.1.2 囊泡的应用............................................................................................21.1.3 囊泡传统制备........................................................................................41.2 电形成法制备囊泡...........................................................................................41.2.1 电形成法简述........................................................................................41.2.2 电形成法制备囊泡的机理.....................................................................51.2.3 电形成法制备囊泡的影响因素.............................................................81.2.4 电形成法制备囊泡的发展.....................................................................91.3 磷脂的掺杂 ..........................................................................................
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