graves病患者外周血cd4cd25foxp3调节性t细胞的检测及意义-detection and significance of cd 4 cd 25 foxp 3 regulatory t cells in peripheral blood of patients with graves disease.docxVIP

Transcriptionfactorforkheadboxp3（Foxp3）.TheFoxp3+Tregisplayaimportantroleinautoimmunediseases.ObjectiveToinvestigatethealterationanditssignificanceofCD4+CD25+Foxp3+TreginpatientswithGravesdisease.MethodTakingtheperipheralbloodfromtheGDpatientsandage-matchedhealthpeople.Peripheralbloodmononuclearcells(PBMCs)werepreparedbyPercolldensitygradient.PBMCsweresurfaceandintracellularstainedinvitro.Finally,stainedcellswereanalyzedbyflowcytometry.ResultsInGDgroup:TheratioofCD4+CD25+Foxp3+TregtoCD4+Tcellinperipheralbloodwerefoundtobesignificantlylowerthanhealthycontrolgroup（P0.05）;theratioofCD4+CD25+Foxp3+TregtoCD4+CD25+Treginperipheralbloodwerealsofoundtobesignificantlylowerthanhealthycontrolgroup（P0.05）.ConclusionThefrequenciesofCD4+CD25+Foxp3+TregdecreasedinpatientswithGDmaysuggestapotentialroleforCD4+CD25+Foxp3+TregintheprogressionandhappenofGD.KeywordsGravesdisease;CD4+CD25+Foxp3+Treg;Flowcytometry英文缩写及中英文对照英文缩写英文全称中文全称GDGravesdisease格里夫斯病TregRegulatoryTcell调节性T淋巴细胞ThhelperTlymphocyte辅助性T淋巴细胞Foxp3transcriptionfactorforkheadboxp3叉头状转录因子P3TSHthyroidstimulatinghormone促甲状腺激素TRAbTSHreceptorantibodies促甲状腺激素受体抗体ILinterleukin白细胞介素IFN-γinterferon-γ干扰素-γTNF-αtumornecrosisfactor肿瘤坏死因子-αCTLA-4cytotoxicT-lymphocyteantigen4细胞毒性T淋巴细胞相关抗原-4GITRglucocorticoid-inducibletumornecrosisfactorreceptor糖皮质激素诱导的肿瘤坏死因子受体FITCfluoresceinisothiocyanate异硫氰酸荧光素APCallophycocyanin别藻蓝蛋白PEphycoerythrin藻红蛋白IPEXimmunodeficiencypolyendocrinopathyandenteropathy,X-LinkedSyndrome免疫功能失调、多发性内分泌病、肠病及X染色体连锁综合征EATexperimentautoimmunethyroiditis实验性自身免疫性甲状腺炎RArheumatoidarthritis类风湿关节炎MSmultiplesclerosis多发性硬化症AITDautoimmunethyroiddisease自身免疫性甲状腺疾病TGF-βtransforminggrowthfactor-β转化生长因子-βPBMCPeripheralBloodMononuclearCell外周血单个核细胞FCMflowcytometry流式细胞术独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行