hif1及ahr信号通路在a549细胞中相互作用的研究-study on interaction between hi f1 and ahr signaling pathway in a549 cells.docxVIP

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hif1及ahr信号通路在a549细胞中相互作用的研究-study on interaction between hi f1 and ahr signaling pathway in a549 cells.docx

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hif1及ahr信号通路在a549细胞中相互作用的研究-study on interaction between hi f1 and ahr signaling pathway in a549 cells

目录内蒙古医科大学学位论文原创性声明中文摘要······························································································（1）英文摘要······························································································（2）论文题目······························································································（4）前言··································································································（4）1实验材料····························································································（5）1.1实验细胞························································································（5）1.2主要仪器························································································（5）1.3主要试剂························································································（5）1.4主要溶液及其配制············································································（6）2实验方法····························································································（9）2.1A549细胞的培养及传代·····································································（9）2.2细胞乏氧微环境的建立······································································（9）2.3实验用B(a)P浓度的选择··································································（11）2.4药物处理A549细胞········································································（11）2.5实时荧光定量PCR法检测VEGF、CAⅨ、CYP1A1和CYP1B1mRNA表达（11）2.6Westernblot法检测VEGF、CAⅨ、CYP1A1和CYP1B1蛋白的表达·········（11）2.7免疫沉淀法检测HIF-1与AhR信号通路的相互作用·······························（13）3实验结果··························································································（14）3.1细胞乏氧微环境的建立····································································（14）3.2实验用B(a)P浓度的选择··································································（16）3.3实时荧光定量PCR法检测VEGF、CAⅨ、CYP1A1和CYP1B1mRNA表达（16）3.4Westernblot法检测VEGF、CAⅨ、CYP1A1和CYP1B1蛋白的表达·········（18）3.5免疫沉淀法检测HIF-1与AhR信号通路的相互作用·······························（20）4讨论·····································