htertcmyc和ki67在卵巢颗粒细胞瘤中的表达及临床意义-expression and clinical significance of htertcmcc and ki67 in ovarian granulosa cell tumors.docx

卵巢颗粒细胞瘤中hTERT，c-myc和Ki-67的表达及临床意义摘要背景与目的卵巢颗粒细胞瘤(granulosacelltumor,GCT)是-种很少见的性索间质肿瘤，占卵巢恶性肿瘤约2％-5％，病因目前尚不清楚。hTERT是端粒酶反转录酶，其表达水平与端粒酶活性有关。c-myc是-种原癌基因，其编码的蛋白质是调节hTERT基因表达的重要转录因子，研究证明，hTERT的上调与多种肿瘤的形成有关。Ki-67是-种细胞增殖核抗原，是反映细胞增殖状态的理想标记物。本研究初步探讨hTERT，c-myc和Ki-67在卵巢颗粒细胞瘤中的表达及临床意义。方法选取35例经病理医师诊断为卵巢颗粒细胞瘤的石蜡切片，用免疫组织化学PV二步法检测其中的hTERT，c-myc和Ki-67表达的变化，并与正常卵巢组织10例比较。结果hTERT和Ki-67的表达在卵巢GCT组高于对照组(P＜0.05)；随临床分期的升高卵巢GCT中hTERT和Ki-67表达水平逐渐升高（P＜0.05）；hTERT的表达随着分化程度的降低而升高（P＜0.05）；Ki-67在肿瘤细胞核分裂像≥3/10HPF组表达高于＜3/10HPF组(P＜0.05)；hTERT和Ki-67表达呈正相关(P＜0.05)。结论hTERT和Ki-67可作为卵巢GCT的辅助诊断指标，测量二者表达可作为判断卵巢GCT的生物学行为的理论依据，而c-myc与OGCT则无明显关系。关键词卵巢颗粒细胞瘤，端粒酶反转录酶，c-myc，Ki-67，免疫组织化学ExpressionofhTERT，c-mycandKi-67inhumanovariangranulosatumorandtheirclinicalsignificanceAbstractBackgroundandObjective:Granulosacelltumoroftheovaryisrareneoplasmsthatoriginatefromsex-cordstromalcells.Theyrepresentabout2-5%ofmalignanttumorsoftheovary.Themolecularpathogenesisofthiskindoftumorisunknown.hTERTishumantelomerasereversetranscriptase,itsexpressionhasrelatedtotheactivityoftelomerase.c-mycprotein，atranscriptionfactorencodedbymycprotooneogene，alsoplayaroleinthepositiveregulationofhTERTexpression.Ki-67isacellproliferationnuclearantigen,whichisrecognizedasamarkerwhichreflectingcellproliferationlevel.ThepurposeofthestudyistodefinetheroleofhTERT，c-mycandKi-67duringtheevolutionofovariangranulosacelltumor.MethodsTheparaffinsectionof35diagnosedasovariangranulosacelltumorwerecollected.ExpressionofhTERT、c-mycandKi-67wasdetectedbyimmunohistochemistry,stainandcomparedwithnormalovariantissue.ResultsTheexpressionofKi-67,andhTERTinOGCTwerehigherthaninnormaltissues.TheexpressionofKi-67andhTERTgraduallyincreasedinclinicalstageⅠ,Ⅱ,Ⅲ.TheexpressionofhTERTgraduallyincreasedwhenthedifferentiationoftissuedecreased.TheexpressionofKi-67intheofkaryokinesis3/10HPFwaslowerthaninkaryokinesis≥3/10HPF.ThereisarelationshipbetweenKi-67andhTERTinOGCT.ConclusionshTERTandKi-67canbetakeasindicatoroftheOGCT,andtheirexpressioncanprovideatheoreticalbasisforpredictin