解磷菌的筛选及生态特性分析-screening of phosphate-solubilizing bacteria and analysis of ecological characteristics.docxVIP

解磷菌的筛选及生态特性分析-screening of phosphate-solubilizing bacteria and analysis of ecological characteristics.docx

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解磷菌的筛选及生态特性分析-screening of phosphate-solubilizing bacteria and analysis of ecological characteristics

结果显示,四株菌在对虾饲料溶液中生长情况良好,菌量均可由103cfu/mL增长到108cfu/mL;提高了对虾饲料活性磷的溶出率,与对照组相比,菌株HY-4可提高活性磷溶出率56.16%,菌株HY-3可提高41.99%,菌株HY-5和菌株HY-6分别可提高16.09%和7.20%。探讨了菌株HY-3和HY-6在模拟池塘环境中的释磷效果。结果显示,菌株HY-3和HY-6均可提高水体中活性磷含量,降低底泥中总磷含量。与对照组相比,水体活性磷含量分别提高14倍和8倍,底泥总磷最高分别降低(9.17±1.40)%和(9.17±4.76)%。关键词:解磷菌,磷,养殖池塘AbstractPhosphorusisanecessarybasicelementforthegrowthofphytoplanktoninaquacultureponds.Inthepondsystem,thephosphorusconcentrationsarehighinthesedimentbutlowintheoverlyingwater.Andtheutilizationofphosphorusintheshrimppondsislow.Phosphate-solubilizingbacteria(PSB)areconsideredtoplayimportantecophysiologicalroleswhichmobilizeinsolublephosphorusinsedimentforphytoplankton.However,theamountsofPSBareataverylowlevel.WhetheritcanimprovetheutilizationrateofphosphorusbyaddingPSBornotbecomethefocusofresearch.Inthisstudy,highefficiencyPSBstrainswereisolated.Andtheadaptabilitiesofdifferenttemperature,salinityandinitialpHonisolatedstrainswerestudied.ThephosphorusreleaseeffectsofPSBonfeedandsimulatedpondswerestudiedtoprovidethetechnicalsupportfortheapplicationofPSBinaquacultureponds.Themainresearchcontentisasfollows.PSBstrainswereisolatedfromthesedimentofponds,thelaboratorystrainsandpurchasedstrainsbypreliminaryscreeningofplateassayandquantitativeassessmentofbrothassay.Thenthestrainswereidentifiedby16SrDNAsequenceanalysisandBiologidentification.TheresultsshowedthatfourstrainsofPSBwereisolatedfromthesedimentofpond,whichwereHY-3,HY-4,HY-5andHY-6.After5dcultivation,theiractivitiesresultedinaccumulationsofsolublephosphate56.85mg/L,64.67mg/L,27.88mg/Land48.34mg/L,respectively.By16SrDNAsequenceanalysisandBiologbiochemicalidentification,thestrainofHY-3andHY-6wereidentifiedasBacillusamyloliquefaciens,HY-4wasidentifiedasPaenibacilluspabuli,andHY-5wasidentifiedasBaciiluslicheniformis.Giventotheenvironmentalconditionsofaquacultureponds,theadaptabilitiesofdifferenttemperature,salinityandinitialpHonstrainsHY-3andHY-6werestudied.TheresultsshowedthatstrainHY-3couldtolerateawiderangeoftemperatureandsalinity,whichcouldg

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