力生长因子e肽(mgf-force growth factor e peptide ( mgf.docxVIP

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力生长因子e肽(mgf-force growth factor e peptide ( mgf.docx

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力生长因子e肽(mgf-force growth factor e peptide ( mgf

ABSTRACTMechano growth factor (MGF) is a hotspot in the research fields of the biomechanics and growth factor recently. It is an alternative splicing variant of Igf-1 gene which is mainly expressed in stretched skeletal muscle, damaged muscle and neuron. Further studies found that MGF and its E peptide (MGF-Ct24E) played an important role in promote muscle hypertrophy, damage repaired and have a function of neuroprotective and myocardial protection. Our previous studies found that MGF mRNA had a high expressed in the cyclic stretched osteoblasts, which suggested that MGF might be a mechano-response molecule in bone and have a role in regulating the proliferation and differentiation of osteoblasts to benefit bone repair. Mechanical stress has an important role in regulating bone growth and reconstruction. Osteoporosis often appeared at the bone fracture site after healing because of the lack of mechanical stress. Therefore, in this study we compared the gene expression profiles of osteoblasts after treat with MGF-Ct24E and mechanical loading by microarray analysis. The aim of this study was to find out the relationship between the treatment of MGF-Ct24E and mechanical loading in bone metabolism, so that we can speculate whether MGF-Ct24E could replace the mechanical stress to benefit bone repair. The main work and conclusions were as follows:The primary osteoblasts were cultured from the calvarias of new born SD rats by tissue piece culture method. The osteoblasts were purified by the difference of the adhesion time with fibroblasts. Osteoblasts were identified by morphology, ALP staining, matrix precursor staining and mineralization staining. The identified results showed that the cells had typical characteristics of osteoblasts. The third generation osteoblasts were used in the subsequent experiments.Cells were suffered with cyclic-strain unit at 12% and 50ng/ml MGF-Ct24E treatment for 8h. Total RNA were extracted, microarray were used to analyze the differentially