利用城市污水培养高脂肪微藻的光生物反应工艺分析-photobiological reaction process analysis of culturing high-fat microalgae by using urban sewage.docxVIP

AbstractUsing urban sewage as medium to cultivate high-lipid microalgae can on the one hand achieve the purpose of the production of bio-diesel. on the other hand, purify the waste water, to achieve its resource utilization. Among this, developing a process with large-scale application prospects and optimizing its corresponding operating conditions is essential. This paper is intended to design a set of continuous operation process in column bioreactor to achieve outdoor high concentration of microalgae culture, to provide a steady stream of raw materials for the preparation of bio-diesel, and efforts resorted to up-to-standard release of water quality index such as nitrogen, phosphorus and other water quality indicators.Learn through literature review and our research group preliminary work, screening the algae species suitable for outdoor sewage training, sewage pretreatment were dicussed. When using actual sewage to culture microalgae outdoors, scenedesmus obliquus shows better growth stability and higher lipid yield than chlorella pyrenoidosa. And the relative abundance of scenedesmus obliquus can reach 93.4%, with strong competitive advantage, more suitable for outdoor sewage culture. After primary sedimentation, the sewage can directly be used for Scenedesmus obliquus outdoor cultivation without autoclaving or sonication treatment.The factors which can affect outdoor sewage cultivation of microalgae were optimized, including light, mix, carbon dioxide enrichment, magnetism and cultivating mode. The smaller the column diameter, the higher the lipid production of Scenedesmus obliquus. And for column photo-bioreactor with large diameter, fiber can be to some extent, improve the internal light conditions, thereby enhancing the production of algae lipid production. Mixing and magnetic treatment can to some extent improve the Scenedesmus obliquus lipid production. When cultivated with 2% carbon dioxide enrichment, Scenedesmus obliquus reach the maximum lipid pr