kim-1在肾小管上皮细胞缺氧损伤中保护作用及hif-1信号通路调节-protective effect of kim - 1 on hypoxic damage of renal tubular epithelial cells and regulation of hif - 1 signaling pathway.docx

FITCFluoresceinisothiocyanate异硫氰酸荧光素HGFHepatocytegrowthfactor肝细胞生长因子HIFHypoxiainducingfactor缺氧诱导因子HOHemeOxygenase血红素氧化酶TIMT-cellimmunoglobulinmucinproteinT细胞免疫球蛋白粘蛋白TIMPTissueinhibitorofmetalloproteinase金属蛋白酶组织抑制剂ICAMIntercellularadhesionmolecule细胞间黏附分子I/RIschemia/Reperfusion缺血再灌注HREHypoxiaResponseElements缺氧反应元件HRPHorseradishperoxidase辣根过氧化物酶KIMKidneyinjurymolecule-1肾损伤分子MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphe3-(4,5-二甲基噻唑基)-2,5-二nyltetrazoliumbromide苯基四氮唑溴化物MAPKMitogenactivatedproteinkinase有丝分裂原活化蛋白激酶ODOpticaldensity光密度PBSPhosphatesalinebuffer磷酸盐缓冲液PCRPolymerasechainreaction多聚酶链式反应PVDFPolyvinylidenefluoride聚偏乙烯氟化物RASRenin-angiotensinsystem肾素-血管紧张素系统rpmRevolutionperminute每分钟转数SDStandarddeviation标准差TrisTris(hydroxymethyl)aminomethane三羟甲基氨基甲烷TUNELTerminaldeoxynucleotidyltransferase脱氧核糖核酸末端转移酶介-mediateddUTPnickendlabeling导的dUTP缺口末端标记法VEGFVascularendotheelialgrowthfactor血管内皮生长因子ProtectiveEffectofKIM-1onRenalTubularEpithelialCellHypoxicInjuryandHIF-1SignalPathwayRegulationAbstractKIM-1(kidneyinjurymolecule-1)isanewlydiscoveredtypeItransmembraneousprotein,whichischaracteristicallyexpressedonthetopmembraneofepithelialcellsofkidneyproximaltubulesfollowinghypoxicinjury.Hence,KIM-1isanidealpremonitionmoleculeforrenalischemic/hypoxicinjury.RecentstudieshaveshownKIM-1tobeafunctionalmolecule,whichcontributestorenalinjuryandrepair.KIM-1isspecificallyexpressedbydedifferentiatedrenaltubularepithelialcells,suggestingthatitmaycontributetodedifferentiation,proliferation,migration,andcontinuousreconstructionofrenaltubularepithelialcells.InvitrostudieshavedemonstratedthatKIM-1isauniquephosphatidylserinereceptor,whichenduesrenaltubularepithelialcellswithphagocytoticfunctionand,thus,helpsscavengeapoptoticandnecroticcellsandrelievetubularobstruction.Nevertheless,theprecisefunctionofKIM-1isnotclearyet,andthereisnodirectevidencethatsupportstheprotectivefunctionofKIM-1.KIM-1isatransmembraneousprotein,anditsextracellularstructureincludesanimmunoglobin-likefunctionaldomainwhichcomprises6cystei