HCV-RNA抗-HCV和ALT联合检测在丙肝中诊断应用.docVIP

HCV-RNA抗-HCV和ALT联合检测在丙肝中诊断应用 　　【摘要】 目的 探讨丙肝(抗-HCV)阳性人群中，其丙型肝炎病毒核酸(HCV RNA)含量与丙氨酸转氨酶(ALT)的关系。方法 采用荧光定量聚合酶链反应(FQ-PCR)技术检测222例丙型肝炎病毒感染者血清HCV RNA含量，采用酶联免疫法检测抗-HCV,采用酶法检测其ALT水平。结果 222例抗-HCV阳性标本中135例HCV RNA阳性(500拷贝/ml)，阳性率为60.8%，120份标本ALT异常(40U/L)，异常率为54%，ALT水平及异常率随HCV RNA含量的升高而增加。 结论 ALT水平及异常率与HCV RNA之间呈正相关，提示ALT水平可间接反映HCV在肝脏的复制程度，HCV-RNA含量与抗-HCV 同时检测可以提高临床对丙型肝炎的诊断，HCV-RNA是反映HCV 复制的可靠指标，结合ALT生化指标结果可帮助临床了解HCV在体内的复制状况。 　　【关键词】 丙型肝炎；丙肝抗体；丙型肝炎病毒核酸 　　 　　Clinical application HCV-RNA anti-HCV And ALT examination in diagnosis of patients with HCV 　　 　　QIAN Ming, YUAN Jun-jun, FU Jian-zhao，et al. Laboratory Department of the Fifth Affiliated Hospital of Sun Yat-sen University,Zhuhai 519000,China 　　 　　【Abstract】 Objective To discuss the relationship between the HCV-RNA levels and ALT levels in the HCV masculine crowd. Methods Fluorescent Quantitative PCR methodswere used to detect the quantify of HCV-RNA in 222 anti-HCV masculine samples, the ALT were detected by automatic biochemistry analyzer. Results In 222 cases anti-HCV masculine samples,135 cases were positive of HCV-RNA and 120 caseswere positive of ALT.Masculine gender rate of HCV-RNA was 60.8%.ALT rate of abnormality was 54%. The ALT level and the rate of abnormality content ascension increased along with the HCV-RNA.Conclusion There was a corrlation between ALT levels and the concentration of HCV RNA. Prompted the ALT level to be possible to reflect HCV indirectly in the liver duplication degree，HCV-RNA levels and anti-HCV detection could improve the clinical diagnosis of hepatitis C, HCV-RNA is a reliable indicator to reflects HCV replication, combined with ALT biochemical results could help clinicians understand the status of HCV replication in the body. 　　【Key words】 HepatitiscHCV-RNA； Anti-HCV；HCV-RNA 　　 　　全球丙肝感染率约为3%，大约117亿人感染了HCV,每年新发丙型肝炎病例315万例［1］。慢性丙肝患者10年内约有20%发展为肝硬化,肝硬化患者每年约有3%发展为肝细胞癌［2］。因此早期诊断和及时治疗尤为重要。HCV-RNA含量与抗-HCV 同时检测可以提高临床对丙型肝炎的诊断，本文检测了222例抗-HCV阳性的血清标本，探讨与HCV-RNA,ALT含量之间的关系。 　　1 资料与方法 　　1.1