TSC1和CDX2蛋白在胃癌中表达及临床意义.docVIP

TSC1和CDX2蛋白在胃癌中表达及临床意义 　　【摘要】目的 检测TSC1及CDX2蛋白在胃癌组织及正常胃粘膜组织中的表达水平，并初步探讨其对于胃癌的临床意义。方法 收集新鲜胃癌及正常胃粘膜组织标本各50例并提取组织蛋白。利用western-blot方法检测TSC1和CDX2蛋白在胃癌组织及正常胃粘膜组织中的表达水平及阳性表达率。结果 在正常胃粘膜组织中均可检测到TSC1蛋白的表达，而检测不到CDX2蛋白的表达。在本实验收集的胃癌组织中TSC1及CDX2蛋白的表达阳性率分别为54%和65%。且表达水平与T分期、分化程度有关。结论 TSC1蛋白及CDX2蛋白在胃癌的发生发展中发挥重要作用。检测二者在胃癌中的表达对于分析胃癌的恶性程度并判断预后有很高的临床价值。 　　【关键词】TSC1蛋白 CDX2蛋白 胃癌 　　中图分类号：R735.2 文献标识码：B 文章编号：1005-0515（2012）2-031-02 　　【Abstract】Objective To detect the expression of TSC1 and CDX2 protein in normal gastric tissue and gastric carcinoma tissue, and to discuss the clinical significance.Methods 50 cases of tissues of gastric carcinoma and normal tissues were collected respectively, and the tissue proteins were extracted. The expression level of TSC1and CDX2 protein and the positivity rate were detected in normal gastric tissue and gastric carcinoma with western-blot. Results The expression of TSC1 protein was detected in normal gastric tissue but CDX2 protein was not detected. The positivity rates of the expression of TSC1 protein and CDX2 protein in gastric carcinoma tissue were 54% and 65%. The expression level of TSC1 and CDX2 protein was associated with T stage and differentiation. Conclusion TSC1 protein and CDX2 protein were closely related with the occurrence and development of gastric carcinoma. To detect the expression of TSC1 and CDX2 protein and to discuss the clinical significance were very important in evaluating the m alignant degree and the prognosis of gastric carcinoma. 　　【Key words】TSC1 protein CDX2 protein gastric carcinoma 　　 　　引言 　　在我国，胃癌的发病率和死亡率均占恶性肿瘤首位。因此，胃癌的早期诊断、确切分期、及时治疗以及判断对治疗的敏感性非常重要。TSC基因即结节性硬化基因是一种肿瘤抑制基因，是首先在结节性硬化综合征(TSC)中发现的，其具有抑制细胞生长的作用，同源异型盒基因CDX2（caudal-related homeobox gene）表达产物广泛存在于胚胎的消化道腺上皮中，CDX2基因的发现源自果蝇，属于DNA结合蛋白大家族，具有维持果蝇形态和肠道发育的作用。在人类，同源异型盒基因不仅控制着胚胎细胞的分化和增殖，而且在成人组织的发育和分化调控中也起着重要作用。Mallo Gv等[1]研究发现CDX2mRNA在85%的结直肠癌中表现出下调，从而推测CDX2确为一种肿瘤抑制基因。近年来，国内外关于CDX2蛋白在胃癌中的研究有一定报道，但结果不一。本研究应用western-blot方法检测TSC1和CDX2蛋白在胃癌组织及正常胃粘膜组织中的表达情况并分析其临床意义。