电针对局灶性脑缺血模型大鼠皮层BDNF、TrkB影响.docVIP

电针对局灶性脑缺血模型大鼠皮层BDNF、TrkB影响 　　摘要：目的：观察电针对不同时间点局灶性脑缺血模型大鼠缺血区皮层BDNF、TrkB的影响。方法：采用线栓改良法复制大脑中动脉缺血模型（MCAO），采用免疫组化SABC法观察缺血皮层BDNF、TrkB表达情况。结果：BDNF、TrkB阳性神经元主要分布在梗死灶周围皮质区。假手术组大鼠相应区域可见少量阳性神经元表达，且强度较弱；模型组各时间点梗死周围皮质BDNF、TrkB阳性神经元比假手术组明显增多，差异有统计学意义（P 　　关键词：脑缺血；电针；风府穴；脑源性神经营养因子（BDNF）；酪氨酸激酶B（TrkB） 　　中图分类号：R285.5；R245.97文献标识码：A文章编号：1673-7717(2012)03-0469-03 　　 　　Influence of Electroacupuncture on the Expressions of BDNF and 　　TrkB in Ischemic Cortex of Rats With Local Cerebral Ischemia 　　 　　WANG Yan?chun1,LIANG Shao?rong1，MA Jun1,GNA Shui?yong1,WANG Shu?ju1, 　　JIANG Cheng?kun1,LEI jun1,WANG Pei?jun1,XU Yong?hai1,CHEN Jian?ming2 　　(1.Hubei University of Traditional Chinese Medicine,Wuhan 430061,China; 　　2.Beijing University of TCM,Beijing 100029,China) 　　 　　Abstract:Objective:To observe the influence of electroacupuncture（EA）on the expressions of BDNF and its receptor TrkB in ischemic cortex of rats with local cerebral ischemia. Methods:The model of focal cerebral ischemia was set up by blocking up the middle cerebral artery(MCA) with filament in rats．The expressions of BDNF and TrkB positive neurons in ischemic cortex was observed with the method of immunocytochemistry SABC. Results:BDNF and TrkB positive neurons were mainly distributed in cortex around the ischemic focus. In pseudo-operation groups ,BDNF and TrkB positive neurons were few and low-expression in identical area.The expressions of BDNF and TrkB around ischemic cortex of model groups were significantly higher than that of pseudo-operation group(P 　　1.2.2行为学检测造模后的动物在自然苏醒后，参照Longa等[7]的5级评分标准对其神经障碍进行评分。淘汰神经功能障碍在0级的动物。 　　1.2.3治疗方法电针组大鼠在行为学检测后立即行电针治疗。取“风府”穴 (参照华兴邦等制定的《实验动物穴位图谱》[8])，用针灸针进针4mm,接G6805-1A型电针治疗仪,选择连续波,频率为20Hz，强度以肢体抖动，且大鼠能安静耐受为度。电极连接方法：正极接风府，负极接耳朵。在每天相同时间点予以电针治疗，每天1次，每次30min，分别连续治疗3、7、14天。假手术组、模型组大鼠在相应时间点只捆绑，不针刺。 　　1.2.4标本采集大鼠腹腔注射10%水合氯醛（4mL/kg）后，快速打开腹腔，暴露心脏，在心尖部剪一小口，将灌胃针插入左心室，剪开右心耳，快速灌注4℃生理盐水，待右心耳处流出澄清液体，换用4%多聚甲醛(pH=7.4)10mL/min灌注至大鼠四肢强直，断头取脑。选取大脑前囟前1mm至前囟后2mm区域脑组织，以确保脑梗死部位包含其中[9-10