哺乳期安赛蜜暴露影响成年小鼠甜味偏好机制初步分析-preliminary analysis of mechanism of acesulfame k exposure affecting sweetness preference in adult mice during lactation.docxVIP

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哺乳期安赛蜜暴露影响成年小鼠甜味偏好机制初步分析-preliminary analysis of mechanism of acesulfame k exposure affecting sweetness preference in adult mice during lactation.docx

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哺乳期安赛蜜暴露影响成年小鼠甜味偏好机制初步分析-preliminary analysis of mechanism of acesulfame k exposure affecting sweetness preference in adult mice during lactation

A preliminary study on the modified sweet preference of adult mice by acesulfame-K exposure during lactationAbstractThe sweet taste preference is plastic. Early taste experience could modify the subsequent sweet taste preference. However the mechanisms of the sweet taste plasticity were still unknown. In this study, we establish a model of sweet preference plasticity development and extensively explore the related molecular mechanisms in the peripheral taste system. In the experiments, maternal ICR mice were drunk with different concentrations of acesulfame-K (AK) solution as drinking water from postnatal day 4 (P4) to P21. The offspring were exposed to AK via mother’s milk. When grew to adult, two bottle preference tests for differet sweet tastants were performed by the offspring mice and prefence thresholds and preference ratios were analysed. Then a proper exposure dose was decided to establish a stable model of sweet taste preference. Based on this model, qRT-PCR, Western blot, immunohistochemistry and ELISA were preformed to explore the modification of the sweet taste related molecular and the morphological characteristics in the peripheral taste system-the tongue and the soft palate.The results of the TBP tests in adult indicated that there were no changes on the preference threshold and preference ratios for AK solution after the early 16 daysexposure (P4-P21) with the concentrations of 0.15 mM and 1.5 mM AK solutions and10 days exposure (P4-P14) with 5 mM AK solution. Early exposure from P4 to P21 with the concentrations of 5 mM, 12.5 mM, 25 mM and 50 mM respectively increased the prefernce thresholds for AK solution and decreased the preference ratios at different concentrations. AK exposure with 5 mM and 12.5 mM AK solution increased the preference threshold to 0.42 mM, compared to 0.13 mM that of the control group. The preference threshold of mice in the 25 mM and 50 mM was improved to 1.33 mM. The preference ratios for AK solution around the preference t