高领类尖柳珊瑚muriceides collaris化学成分及生物活性再分析-re-analysis of chemical constituents and biological activities of muriceides collaboration is of high-necked corallium acuminatum.docx

摘要 摘要 PAGE PAGE 2 经手性拆分进一步纯化得到 (+)-12 和 (?)-12? ； 7 个愈创木烷型倍半萜： 3-formyl-5-isopropyl-8-methylazulene (14) ， 1-methoxylguaiazulene (15) ， guaiazulene-1, 7-dione (16) ， 1-formylguaiazulene (17) 、 guaiazulene (18) ， sesquiterpene ketolactone (19)、guaianediol (20)；1个苯衍生物：对羟基苯甲醛(23)； 3个核苷：脱氧腺嘌呤核苷 (24)，脱氧尿嘧啶核苷 (25)，脱氧胸腺嘧啶核苷 (26)； 1个嘧啶类化合物：尿嘧啶 (27)。其中化合物1~12、21~23为首次从柳珊瑚 Muriceides Collaris中分离得到。 生物活性研究：选择K562(人红白血病细胞)、HeLa(人宫颈癌细胞)、HL-60(人 白血病细胞)和A549(人肺癌细胞)4种肿瘤细胞株，以阿霉素为阳性对照，采用 MTT法或SRB法对大部分单体化合物的体外抗肿瘤活性进行了初步评价，测试结 果表明，化合物7对K562细胞显示较强的细胞毒活性，IC50值分别为8.37μM，化 合物(?)-8对HL-60细胞显示较强的细胞毒活性，IC50值分别为5.08μM，化合物11 对HeLa细胞显示较强的细胞毒活性，IC50值为5.25μM。以利巴韦林为阳性对照， 采用CPE+MTT法，测试了部分单体化合物的抗病毒活性，结果表明，化合物(+)-5 显示中等强度抗病毒活性，化合物(+)-2和(?)-6显示弱抗病毒活性。 此外，根据茚类倍半萜(1~6，13)和愈创木薁倍半萜生物碱(7~9)的结构特点， 并结合文献，分别推测了其可能的生物合成途径。 关键词：高领类尖柳珊瑚；化学成分；对映异构体；抗肿瘤活性；抗病毒活性 Abs Abstract Re-study on Chemical Constituents and Bioactivities of the Gorgonian Muriceides collaris Abstract A gorgonian, commonly known as sea fan or sea whip, is a sessile animal of the Subclass Alcyonaria, Class Anthozoa, Phylum Coelenterata, found throughout the oceans of the world. There are 45 species from seven families of gorgonians in China, mainly located in Guangdong, Hainan, Zhejiang and Taiwan coast. The South China Sea contains plentiful of marine organisms, notably gorgonians. The gorgonian Muriceides collaris with beautiful sky-blue , were collected from the coast of Weizhou Island, Guangxi province, Sourth China Sea in October, 2010. At present, there were few reports on the chemical constituents of Muriceides collaris. In our ongoing program to search for natural products with unique structural features and pronounced biological activities, we have performed a systematic investigation on the chemical constituents and biological activities of the gorgonian Muriceides collaris again on the basis of the first study on this species of gorgonians by a postgraduate student Xuefeng Shi of the class of 2010. . Isolation and struc