液相和凝胶相单层磷脂膜在还原氧化石墨烯表面自组装-self-assembly of liquid phase and gel phase monolayer phospholipid films on the surface of reduced graphene oxide.docxVIP

脂膜的侧向流动性。本工作以还原氧化石墨烯引导磷脂分子自组装为例，证明了固体基底表面的局域结构及物理化学性质对引导生物膜组装及其相行为具有极 其重要的影响。关键词：单层磷脂膜；界面相互作用；液相；凝胶相；还原氧化石墨烯； 自组装特性作者：芮龙飞 指导教师：元冰Reduced graphene oxide directed self-assembly of phospholipid monolayers in liquid and gel phasesAbstractCell membranes play an important role in intercellular substance transport and signal and power transmission. The response of cell membranes to the local physical environment significantly determines these biological processes and the practical applications of biomaterials. A better understanding of the dynamic assembly and environmental response of lipid membranes can help understand these processes and design novel nanomaterials for biomedical applications. In this work, we take advantage of AFM technique to investigate the directed self-assembly of phospholipid molecules, in both liquid and gel phases, in response to the surface features of monolayered reduced graphene oxide (rGO) sheets that are supported on a mica surface. Results show that subtle structural details in rGO substrate are amplified into dramatic differences in morphology and lateral fluidity in supported lipid monolayers. This work provides a new framework for understanding the cell membrane responses to rGO and help design novel rGO/lipid nanocomposite biomaterials.This thesis includes: (1) monolayered graphene oxide and reduced graphene oxide sheets were fabricated on mica surface; (2) phospholipid molecules, in both liquid and gel phases, were assembled on the rGO surface by the Langmuir-Blodgett (LB) method. The surficial property of them was characterized with atomic force microscope (AFM). (3) Through reducing the surface pressure of the phospholipid membrane, the assembly mechanism of the lipid molecules was investigated; (4) we further increased the temperature to above the phase transition point of the lipid to learn about the influence from the lipid-rGO interaction on the lateral fluidities of the lipid membrane. The results indicate that the hydrophobic a