中药复方促使T淋巴细胞增殖实验研究.docVIP

中药复方促使T淋巴细胞增殖实验研究 　　【摘要】 目的 研究中药复方促使T淋巴细胞增殖。方法 从SD大鼠血液里分离淋巴细胞，进行体外培养。设正常细胞对照组、阳性药物对照组、转移因子组、复方中药高剂量组、中剂量组、低剂量组。培养1 d后把药加入培养液中，继续培养2 d，加Am-Blue细胞增殖与活性检测试剂，避光培养4 h后，通过酶标仪检测，分析淋巴细胞的增值情况。结果 中药复方高、中、低剂量组OD值均明显增高，与空白组比较P＜0.01；与阳性药物对照组比较，P＜0.01；高剂量组与中、低剂量组比较P＜0.01；转移因子组与空白组比较P＜0.01。结论 中药复方能促使T淋巴细胞增殖，且与剂量有关。 　　【关键词】 中药复方；T淋巴细胞；增殖 　　 　　Study on compound traditional chinese medicine promote toT-lymphocyte proliferation experiment 　　ZHANG Hui, LI Yu-hu. Shanghai University of Traditional Chinese Medicine 　　Shanghai201203,China 　　 　　【Abstract】Objective Study of compound traditional Chinese medicine can promote to T-lymphocyte proliferation. Methods Separate T-lymphocyte from blood of SD rat, culture in vitro. Install normal cell control group, active-drug control group, transfer factor group, compound traditional Chinese medicinehigh dose group、middle dose group，low dose group. Give the medicine to culture medium after culture one day. Continue to culture 2 days and take Am-Blue into 96 orifice plate, culture 4 hours in dark. Use enzyme-labelling measuring instrument to measure after three days. Analyse proliferation of T-lymphocyte. Results OD of compound traditional Chinese medicine high dose group、middle dose group、low dose groupcan improve obviously. Theirs compare to blank control group P＜0.01; theirs compare to Active-drug control group P＜0.01; compound traditional Chinese medicinehigh dose group compare to middle dose group、low dose group P＜0.01. transfer factor group compare to blank control group P＜0.01. Conclusion Compound traditional Chinese medicine can promote to T-lymphocyte proliferation, and relation to dosage. 　　 【Key words】Compound traditional Chinese medicine; T-lymphocyte; Proliferation 　　 　　转移因子(Transfer factor TF)是从致敏动物的白细胞或脾脏、淋巴结等免疫器官中提取的一种低分子量多肽―核苷酸复合物，因其能将供体某种特定的细胞免???功能特异地转移给受体，故称为转移因子。目前对转移因子的研究很多，本研究通过转移因子与中药的复方来研究中药复方对体外培养的大鼠淋巴细胞的影响作用，分析复方转移因子各种组别对细胞增殖的影响及与剂量的关系，为后续的体内试验提供参考依据，探讨复方中药免疫增强作用和对复方转移因子的临床应用开发奠定基础。 　　1材料与方法 　　1.1 实验材料 　　1.1