联用尼美舒利对—氟尿嘧啶在大鼠体内药代动力学的影响.docxVIP

  • 9
  • 0
  • 约1.79万字
  • 约 37页
  • 2018-06-04 发布于江西
  • 举报

联用尼美舒利对—氟尿嘧啶在大鼠体内药代动力学的影响.docx

联用尼美舒利对—氟尿嘧啶在大鼠体内药代动力学的影响.docx

administered5一Fuwithipheparinizedtubesbeforeandatat5day.Carotidbloodwascollectedinto1,3,5,10,20,30,60,90,120minafterip5--Fuphysiologicalsaltsolution(48mg?kg一1).Plasmawassepara—tedbycentrifugationfor15min(3000r?min-1).Thespecimenswerestoredatrefrigeratoruntiltobeanalyzed.3.DataanalysisThepharmacokineticparameterswerecalculatedby3p97softwaredevelopedbytheChinesePharmacologicalSociety.AndthecorrelationmongAUC,C。。anddosesweredeterminedbylinearregression.ResultsIdentificationofdeterminationof5一flurouracilinplasmaChromatogramsof5——flurouracilandthespecifitya—Theretentiontimeof5一flurouraciliS(4,0750.008)minandreten—tiontimeof5一BruinternalstandardiS(7.4500.020)min.Plasmaen—dogenousmaterialshasnointerferencewiththecontentof5--flurouracil.Standardcurve5——Fuofvariousknownconcentrationwasaddedtoplasmaatthefi—.nalconcentrationof40,20,10,5,2.5,1.25/*g?mL~.Theywerepro—cessedaccordingtotheaboveprocedure.Thelinearregressionwascon—structedbyusingpeakareacomparisonofthe5--Fuand5——Bruandcor?-respondingconcentration.Satisfatorylinearitywasobservedintherangeof1.25~80”g?mI,ofthedrug.TheregressionequationwasY一0.2103+0.0537X(r一0.9996,n一7)。Inthisequation,Yisthepeakareacomparison,Cistheconcentration(肚g?mL_1)ofthedruginplasmaandristhecoefficientofcorrelation.Thedetectablelimitof5一Fuundertheseconditionswas0.625pg?mL~.1.3Relativerecoveryandintra--day,inter--dayprecisionPlasmasamples(n一5)withthefinalcontentrationsof40,10,l-25”g?ml1of5一FuwereextractedaccordingtOtheproceduremen—tionedabove.Thesesamplesweretreatedaccordingtotheaboveinter一?5’dayandwithin--dayrepeatedly.Therecoveryofthecompoundwascal—culatedbycomparingtheexperimentalvaluewiththecorrespondingtheo—reticalvalue.Themeanrelativerecoveryof5一Fuwasabove90%,Thecoefficientsofvariationofwithin—dayandinter—daywerebothlessthan20%.StudyonPharmacokineticsConcentrationof5——FuinbloodafteripThesamplesweretreatedandthecorrespondingconcentrationsinbloodwerecalculated.Thelevelofthedrugdeclinedwithtimeinthetwocompartmentpattern.PharmacokineticparametersThe

文档评论(0)

1亿VIP精品文档

相关文档