局灶性脑缺血预处理早期抑制脑缺血耐受大鼠半暗带区argrs基因和蛋白的表达-early inhibition of arg rs gene and protein expression in the penumbra of cerebral ischemia-tolerant rats by focal cerebral ischemia preconditioning.docxVIP

局灶性脑缺血预处理早期抑制脑缺血耐受大鼠半暗带区ArgRS 基因和蛋白的表达摘要目的 观察缺血预处理所产生的脑缺血耐受大鼠脑梗塞缺血半暗带 ArgRS 基 因和蛋白的表达变化。方法 65 只大鼠随机分为正常组（5 只）、缺血预处理后脑梗塞组（30 只）、 脑梗塞组（30 只），后两组又各分为假手术组、2h、6h、12h、24h、48h 六个亚 组各 5 只，采用改良 Longa 线栓法制作大鼠局灶脑缺血模型。在相应时间点进行 神经功能缺损评分，根据评分筛查模型，手术显微镜下取梗塞半暗带的皮质脑组 织标本进行 RT-PCR 及 Western-blot 测定。结果 缺血预处理后脑梗塞组在不同时间点的 ArgRS 基因及蛋白表达均显著 低于脑梗塞组（P0.05）。正常组，缺血预处理后脑梗塞假手术组及脑梗塞假手 术组间 ArgRS 基因及蛋白表达差异无统计学意义。结论 脑缺血预处理后所产生的脑缺血耐受现象早期可能存在通过抑制ArgRS 基因及蛋白表达来发挥其神经保护作用。【关键词】 缺血耐受；ArgRS；神经保护Focal Celebral Brain Ischemic Preconditioning,(BIP) Inhibit the Expressions of ArgRs Gene and Albumen of Rat Model of Brain Ischemic Tolerance Semidarkness Zonary in the Early PeriodAbstractObjective To investigate the changes of brain Ischemic tolerance semidarkness zonary ArgRS gene and albumen which were caused by brain ischemic preconditioning.Methods 65rats were randomly assigned into 3groups: normal (n=5), ischemic preconditioning cerebral infarction (n=30), cerebral infarction (n=30). The latter twogroups were respectively divided into 6 subgroups: sham surgery, 2h、6h、12h、24h、48h with 5 rats in each group. Then make the rat focal brain Ischemic model by adopting improved Longa’s obstruction method. Investigate the neurologic impairment at the corresponding time points and assay RT-PCR and Western-blot of infarction semidarkness zonary brain tissue samples taken under the operating microscopes.Results The expressions of ArgRs Gene and albumen of ischemic preconditioning cerebral infarction were all significantly lower than those of cerebral infarction at different time points (p0.05). ArgRs Gene and albumen had no differences in thethree groups：normal, sham surgery of ischemic preconditioning cerebral infarctionand sham surgery of cerebral infarction.Conclusion The nerves protective effect of brain ischemic tolerance developed by inhibiting the expression of ArgRs gene and albumen may exist in the early period of brain ischemic tolerance caused by brain ischemic preconditioning.Key words: Ischemic Tolerance;