阿司匹林对肠上皮细胞株Caco-2凋亡影响.docVIP

阿司匹林对肠上皮细胞株Caco-2凋亡影响 　　【摘要】目的 探讨阿司匹林对肠上皮细胞株Caco-2的生长抑制作用及对细胞凋亡的影响。方法 将不同浓度的阿司匹林溶液(5、10和20mmol/L)与肠上皮细胞株Caco-2共同培养24 h、48h和72h，采用MTT法检测阿司匹林对Caco-2细胞增殖的作用。倒置相差显微镜观察各浓度组阿司匹林与Caco-2细胞共同培养24h后细胞形态学变化。流式细胞术膜联蛋白V-异硫氰酸荧光素-碘化丙啶（Annexin V FITC/PI）双染法检测各浓度药物组与Caco-2细胞共同培养24h后对细胞凋亡的影响。结果 在5、10和20 mmol/L阿司匹林作用下，24h、48h和72h测细胞存活率分别为84.32%±1.29%、76.89%±2.28%和68.74%±2.05%；62.33%±2.02%、50.28%±0.98%和41.46%±1.52%；42.99% ± 2.09%、32.66%±1.99%和20.38%±0.67%。光镜下可见药物作用后贴壁细胞数量减少，细胞变圆，悬浮，部分细胞核浓缩，细胞损害随药物浓度增加而增大。流式细胞术显示：对照组凋亡率为3.8％，5mmol/L阿司匹林组凋亡率为12.7％，10mmol/L阿司匹林组凋亡率为44.7％，20mmol/L阿司匹林组凋亡率为58.1％。随着阿司匹林药物浓度增加，Caco-2细胞的凋亡率亦随之增加。结论 阿司匹林可抑制肠上皮细胞的增殖，呈现剂量-时间依赖性，诱导肠上皮细胞发生凋亡。 　　【关键词】阿司匹林 Caco-2细胞 MTT 细胞凋亡 　　中图分类号：R96文献标识码：B文章编号：1005-0515（2011）12-072-02 　　Effects of aspirin on the apoptosis of enterocytes Caco-2 　　SUN Yi1 ZHANG Zhenyu2 ZHU Jiayi1 YANG Bin1 　　（1. Department of Gastroenterology, Taizhou People’s Hospital, Taizhou 225300, China； 　　2. Department of Gastroenterology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing 210006, China） 　　【Abstract】Objective To investigate the effect of aspirin on the proliferation and apoptosis of enterocytes. Methods Caco-2 cells were cultured in vitro. After co-culture of aspirin solutions of 5, 10 and 20 mmol/L with cells for 24h, 48h and 72h, the survival rates of cells in each group were examined using MTT assay. Caco-2 cells morphology change after drug action was observed by inverted phase contrast microscope. Flow cytometry was used to detect cells apoptosis after co-culture of aspirin solution with Caco-2 cells for 24h. Results After 24h, 48h and 72h，the survival rates of Caco-2 cells were 84.32%± 1.29%, 76.89%±2.28% and 68.74%±2.05%；62.33%±2.02%, 50.28%±0.98% and 41.46%±1.52%；42.99%±2.09%, 32.66%±1.99% and 20.38%±0.67% in groups with aspirin of 5, 10 and 20 mmol/L, respectively. Caco-2 cells injury was induced by aspirin in a concentration-dependent and time-dependent manner. The number of adherent cells was reduced,