高住低练大鼠肝细胞凋亡与bax,bcl-2和HIF-1α之间关系.docVIP

高住低练大鼠肝细胞凋亡与bax,bcl-2和HIF-1α之间关系 　　摘 要:探讨“高住低练”肝细胞凋亡与凋亡调控基因(包括HIF1α)之 间关系,为低氧训练提供科研资料。方法:健康成年雄性SD大鼠60只,随机分为6组。安静 对照组(C)不训练,不进行低氧暴露;低氧暴露组(HE)和高住低练组(Hilo)每天低氧 暴露(氧浓度12.6%,相当于海拔4 000 m)8 h和12 h,5 d/周,共4周;常氧运动组和高 住低练组每天均以25 m/min的速度训练1 h,5 d/周,共4周。采用免疫组织化学方法和计算 机显 微图像分析系统分析bax、bcl2和HIF1α阳性表达、阳性物质的定位及定量。结果:1)与C组相比实验各组细胞凋亡指数著性升高(p 　　关键词:高住低练;肝细胞凋亡;凋亡调控基因;低氧诱导因子1α 　　中图分类号:G804.7文献标识码:A文章编号 :1007-3612(2010)01-0062-05 　　 　　Relation of Living High/Training Low on Hepatic Apoptosis betwee n Bax, Bcl2 and HIF1α in 　　SpragueDauley Rats 　　LIU Wenfeng1, LU Jianqing2, QU Shulin1, TANG Changf a1, LI Jianghua3 　　(1.Hunan Normal University, Changsha 410012, Hunan China; 2. Xi angnan College, Chenzhou 423000, Hunan China; 　　3. Jiangxi Normal Universi ty, Nanchang 330027, Jiangxi China) 　　Abstract: This research studies hepatic apoptotic gene, and applies research dat a for hypoxic training in sports practice applications. It attempts to explore t he relation between apoptotic gene and apoptosis on living high/training low. 60male SD rats are randomly divided into six groups. Group C do not hold hypoxiaand training. Groups HE and Hilo are held in hypoxia chamber (12.5% concentratio ns of oxygen, simulated as a altitude of 4 000 m) with 8h and 12h each day, 5 da ys per week for 4 weeks. Groups T and Hilo are trained at the speed of the 25 m/ min for 1 hour each day, 5 days per week for 4 weeks. Immunohistochemical and co mputer image processing technique are applied to detect positive expression andquantificational analysis for HIF1α,bax and bcl2 protein. As compared withgroup C, the index of apoptosis increases significantly in other experimental g roups(p 　　Key words: living high/training low; liver apoptosis; apoptotic gene; HI F1α 　　 　　肝细胞凋亡是个多基因调控的复杂过程,其产生的机制至今尚未完全清楚。目前的研究 多与bcl-2家族有关,bcl-2/bax和bax/bal-xl可抑制细胞凋亡,bax/bcl-2、bax/bax和bax/ bal-xl可促进细胞凋??[1-4]。bax的作用与bcl-2相反,促进细胞凋亡,许多细胞 的凋亡过程中常伴有bax表达水平的升高,如Kobayashi等[5]转染bax时发现不仅能 够诱导许多细胞发 生自发凋亡,而且可以促进多种因素诱导的多种细胞凋亡。