高压氧对快速扩张皮肤血流影响实验研究.docVIP

高压氧对快速扩张皮肤血流影响实验研究 　　[摘要]目的:探讨高压氧对快速扩张皮肤血流量的影响。方法:以新西兰大白兔为动物模型,实验分为3组:①高压氧治疗快速扩张组(A组);②单纯快速扩张组(B组);③仅埋置扩张器不扩张的对照组(C组)。测量各组术前、术后、注射盐水前后及高压氧治疗结束后即刻血流量及皮肤温度的变化,进行比较和统计学分析。结果:术前A组扩张区域皮肤血流量为93.32±11.93pu,B组为96.35±14.31pu,扩张前两天,两组血流量无显著性差异(P0.05),扩张第四天至扩张结束A组扩张皮肤血流量较B组多(P 　　[关键词]快速扩张;高压氧;血流灌注量 　　[中图分类号]R622[文献标识码]A[文章编号]1008-6455(2010)02-0222-03 　　 　　The influence of HBO therapy on blood flow of rabbit skin in rapid expansion:A randomized controlled experimental study 　　JU Zhao-yu1,FENG Xing-hua1,GUAN Hao2,ZHANG Jun-rui1 　　(1.Department of Oral and Maxillofacial Plastic Surgery,College of Stomatology,the Fourth Military Medical University,Xian 710032,Shaanxi,China;2.Department of Burns Surgery,Xijing Hospital,Fourth Military Medical University,Xi an 710033,Shaanxi,China) 　　 　　Abstract:ObjectiveThe study was to investigate the influence of hyperbaric oxygen (HBO) therapy upon the blood flow of rapid expanded skin.MethodsA total of 18 New Zealand male rabbits were divided randomly into three groups: ①group A were treated by HBO therapy after expander inflation everyday.②group B were not treated by HBO therapy after expander inflation.③group C were not inflated as the negative control group. The blood flow and skin temperature of the expanded areas were examined before and after operation, inflation and HBO therapy.ResultsThe blood flow of group A was 93.32±11.93pu on average before operation, and group B was 96.35±14.31pu.At the first two days after operation there was no significant difference (P0.05)between the two groups, but after that, the blood flow of group A became much higher than that of group B (P 　　1.2 主要仪器及试剂:50ml长方形硅胶皮肤扩张器(上海威宁整形制品有限公司)18个,DWC450-1150型动物实验专用高压氧舱(上海七零一所杨园医用氧舱厂),激光多普勒血流灌注成像仪PeriScan PIM 3(瑞典百灵威医疗仪器公司),红外线温度计(滨州泰乐益康科技有限公司),速眠新Ⅱ(北京宠必威生物科???有限公司)。 　　1.3 实验方法及手术操作:制作一个与扩张器大小形状相同的塑料片,速眠新Ⅱ肌注麻醉后,在家兔背部右侧前后肢间,距背部正中线2cm处沿塑料片边缘用蓝色标记笔标记一圈,作为扩张器植入区。在家兔背部左侧距正中线1cm平行于背部正中线作一长2cm的切口,深达肌肉,沿肉膜层与肌肉之间分离间隙,因扩张器较小,我们选择采用文献[6]中的背部切口皮肤外缝法以限制周围皮肤向扩张区