三七皂甙对心肌腺的苷三磷酸酶的影响.doc

三七皂甙对心肌腺苷三磷酸酶的影响 BIBI,ID:1ssctaPharmaco[ogicaSinica中国药理 EffectsofPanaxnotoginsengsaponinson myocardialadenosinetriph0sphatase CHENJi—Qiu.ZHANGYue—Guang,LISheng—Iinn,ZENGQing,RONGMingZhi (DepartmentofPathophysioloRy.GuilinMedicalCollege,Guilin541001?China) ABSTRACTEffectsoftota1Pan(1oTnotogin sengsaponins(PNS)andPanaxnotoginseng saponinmonomersRb1andRgL(Rb1.Rg1)on totalATPaseandNa一K一一exchanging ATPaseofguineapigheartwerestudied.It wasfoundthatPNSinhibitedthetotalmy ocardia1ATPase,buthadnosignificanteffect onthemyocardla[Na一K一exchanging ATPase.Thetota1ATPasewasinhibitedby Rg1,andmoreclearlybyRb1.Theauto maticityandcontractilityofisolatedguineapig atria-44eceinhibitedbyRb1.Rgldecreased thespontaneousfrequencyofisolatedguinea pigrightatrium,butnotmarkedlythecon tractilityoftheIeftatrium.Theseresults demonstratethatRh】isthemainingredientin PNS. KEYWORDSginseng;saponins;Na一 K…exchangingATPase;adenosinetriphos phatase;myocardialcontraction;heartatrium TotalPanaxnotoginsengsaponins(PNS) couldinhibitthemyocardia1automaticity?and contractility,antagonizethecentralandpe rlpheralarrhythmias.impairthepositivein— otropicactionandtoxicreactionofouabain andblocktheslowcalciumchannelsI1s?In thispaper.westudiedtheeffectsofPNS,Rbl andRg1(purifiedsaponinsfromP ontoginseng)onIota1myocardia1ATPaseand Na4-K一ATPase.andalsotheeffectsofRb, andRg1onautomaticityandcontractilityof Receivedl9930261AccepteProjectsupportedbytheMedLca[ResearchFoundation.f Departmetu0EPubLicHeaLth0f(;u~agxi,9105. isolatedguineapigatriatoexploretheiractive constituents. MATERIALSANDMETHoDS DrugsPNS(YunnanPhytopharmaceuticalFac— tory).RhLandRg(DivisionofPlantChemistry,Kun— mingMedica]College),ATP-Na2(ShanghaiBiochemi catReagentFactoryouabain(Oua)andbovine serumalbumin(BSA)(Sigma).Otherreagentswere allARandwerepreparedwithredistilledwater. PreparationofmyocardialhomogenateGuinea pigsofeithersex(300±s10g)were~~unned,T heart8靴reexcisedandwashedinsaIineTheventri deswerecutandhomogenizedin10m】TrisHC】 bu