细菌鞭毛染色培养条件研究.docVIP

细菌鞭毛染色培养条件研究 　　王 燕1 曾 燏2 　　1.川北医学院病原生物与免疫学教学实验中心，四川南充 637000； 　　2.西华师范大学生命科学院，四川南充 637002 　　[摘要] 目的 比较细菌在不同培养基和不同菌龄条件下鞭毛的染色效果，探索细菌鞭毛染色形态鉴定理想的培育基和培育时间。 方法 ①将普通变形杆菌分别接种在肉汤培养基、琼脂培养基、血琼脂培养基3种不同培养基内，37℃培养18 h后，染色镜检，选择取出染色效果最好的理想培养基；②将普通变形杆菌接种到理想培养基上分别培育8、12、18、24 h不同时间后，染色镜检，选择出细菌鞭毛染色效果最好的培育时间。 结果 ①血琼脂中培养的细菌鞭毛结构清晰、形态完整、背景干净，镜检效果最好；②12～18 h菌龄的细菌菌体中等大小，鞭毛清晰完整，镜检效果好。 结论 血琼脂是鞭毛染色教学和形态鉴定理想的培养基；12～18 h是细菌在血琼脂培养基中的最佳生长时间。 　　[关键词] 鞭毛染色；培养基和培养时间；镀银染色；普通变形杆菌 　　[中图分类号] R446 [文献标识码] A [文章编号] 1673-7210（2014）03（b）-0035-03 　　The study of culture conditions in bacterial flagella staining 　　WANG Yan1 ZENG Yu2 　　1.Experimental Teaching Center for Pathogenic Subject， North Sichuan Medical University， Sichuan Province， Nanchong 637000， China； 2.College of Life Science， China West Normal University， Sichuan Province， Nanchong 637002， China 　　[Abstract] Objective To compare the bacterial flagella staining effect in different medium and different age conditions， and to explore the best medium and incubation time for bacterial flagella staining teaching and morphological identification. Methods ①The Proteus vulgaris were inoculated in broth medium， agar medium， and blood agar medium 3 different media， cultured for 18 hours based on 37℃， then stained and checked with the microscopic， and the best medium was chosen. ②The Proteus vulgaris were inoculated into the ideal medium culture for 8， 12， 18， 24 h respectively， then stained and checked with microscopic， and the best time of cultivation in flagella staining effect was selected. Results ①The bacterial growth on blood agar had good results， with complete structure， clear morphological and clean background. ②12-18 hours age had the best effect with clear and complete flagella. Conclusion The blood agar is the best medium for bacterial flagella staining teaching and morphological identification. Meanwhile， the best time of bacteria in culture is 12-18 hours. 　　[Key words] Flagella staining； Media and incubation time； Silver staining；