血吸虫病和激动的肠炎的诊断和流行病学的课件.ppt

In Egypt, schistosomiasis ( from S. haematobium and mansoni)has been strongly linked to bladder cancer. The evidence includes: Epidemiological evidence Experimentally induced schistosomiasis Histopathology Age and gender ratios Colorectal cancer correlates with prevalence and intensity of shistosome infection. The age of onset of cancer is lower in areas of shcistosome endemicity and length of survival is shorter. Affected individuals usually have a long history of inflammatory large bowel symptoms. The cancers correspond in anatomic location to the area most affected by the parasite. Tests for Fecal Occult Blood Chemical Tests Principle: Based on peroxidase activity of heme. Advantages: Sensitive, Inexpensive. Will detect digested blood. Quantitation possible. Disadvantages: Not specific. Dietary restriction required. Immunological Tests Principle: Based on detection of human hemoglobin. Advantages: Very specific. No dietary restrictions. Disadvantages: Expensive. More complex to perfom. Examples of Chemical Tests Chromogens: O-tolidine/Benzidine, Guaic, Pyramidon. Example of Immunological Test A comparison was made among four neutrophil-derived proteins in stool as an indicator of disease activity in ulcerative colitis. There is a need for a rapid test for lactoferrin. An immunochromatographic test using polyclonal antibodies: a sandwich immunoassay with colloidal gold as visualization reagent. Instruments which allow for quantitation Instruments which allow for quantitation Flow through membrane test for detecting S. japonicum antibodies Flow through membrane test for detecting S. japonicum antibodies Flow through membrane test for detecting S. japonicum antibodies * * The Association of Schistosomiasis and Inflammatory Bowel Disease with Colorectal Cancer: Tools for Diagnosis and Epidemiology Peter Chun, Ph.D 天马行空官方博客：/tmxk_docin ；QQ:1318241189；QQ群：175569632 M. H. Mostafa et al. Relationship between Schistosomiasis and Bladder Cancer. Clin Microbiol R