苯妥英钠对缺血再灌注脑损伤保护作用的实验分析-experimental analysis of the protective effect of phenytoin sodium on cerebral ischemia-reperfusion injury.docxVIP

山西医科大学2003硕士研究生毕业论文苯妥英钠对缺血再灌注脑损伤保护作用的实验研究摘要 目的：进一步探讨苯妥英钠对缺血再灌注脑损伤保护作用的机 制，进一步拓展临床应用。 方法：采用夹闭大鼠双侧颈总动脉的急性前脑缺血再灌注模型， 40只雄性Wistar大鼠，随机分组：A组假手术组、B组生理 盐水对照组、C组苯妥英钠给药组(15mg／kg组)、D组苯妥英 钠给药组(30mg／kg组)。观察脑组织超微结构的改变，脑组织 ATP酶活性、脑细胞线粒体琥珀酸脱氢酶活力及缺血脑组织Fas 阳性细胞的表达，并观察使用苯妥英钠后对上述指标的影响。 结果：超微结构可以看出，C、D组可明显改善脑组织病理改变， 各项指标结果表明，c、D组同B组比较，可以显著提高ATP酶 活性(PO．05)，提高线粒体琥珀酸脱氢酶活力(P0．05)，而 两项指标C、D组差异无显著性(p0．05)，并且C、D组Fas阳 性细胞的表达数明显减少，同B组比较差异有显著性(PO．01)。 结论：(1)苯妥英钠对脑缺血再灌注脑损伤有保护作用；(2)苯 妥英钠拮抗缺血再灌注脑损伤的机制与其钠离子通道阻滞、增加 皮质的ATP酶活性、减少兴奋性毒性、保护线粒体功能等多种机 制有关。关键词苯妥英钠缺血再灌注脑损伤脑保护ATP酶琥珀酸脱氢酶Fas生曼垦苎苎兰 垫!!塑主竺壅生望些丝查Experimental Research of Protective Effect of Phenytoin Sodiumon onCerebral lschemia—Reperfusion of RatsABSTRACTOBJECTIVE：Probing into the mechanism ofPhenytoin Sodium protective effect on brain tissue in cerebral ischemia reperfusion． METH ODS：Rats were subjected to cerebral ischemia by bilateralligation of the common carotid arteries for 45 minutes and thenreperfusion for 2 hours．Forty rats were randomly divided into four groups：sham group(group A)tmdergoing all surgical procedures but ischemia；normal saline group(group B)；phenytoin(15mg／kg)group (group C)；phenytoin(30mg／kg)group(group D)．After completingthe required procedures，the samples of brain tissues were taken tomeasure the activities of ATPase and mitochondrial succinic dehydrogenase(SDH)、to observe the expression ofFas protein and the ultrastructure of cortex，furthermore phenytoin’S influence Oil above indexes was observed．RESULTS：We Can see from all indexes．the activities ofATPase and SDH was significantly inhanced in group C and D compared with group B(p0．05)，and there was no statistic difference between group C and D；and the the expression of Fas protein was significantly reduced in group C and D compared with group B山西医科大学 2003硕士研究生毕业论丈(p0．01)．And the ultrastructure of cortex was improved in group C and D．CONCLUSION：(1)Using Phenytoin Sodium have protectiveeffect on brain tissue in cerebral ischemia reperfusion，and there isno statistic dif