不同铂类对cne-2细胞株放射增敏的体外比较实验研究-comparative study on radiosensitization of different platinum species to cne - 2 cell line in vitro.docxVIP

Comparative experimental study of the radiosensitization effect of different platinum compounds on CNE-2 Nasopharygeal Carcinoma cell line in vitroAbstract：Objective：To study and compare the radiosensitization ofdifferent platinum compounds, Cisplatin, Carboplatin, Oxaliplatin and Nedaplatin on CNE-2 human nasopharygeal carcinoma cell line in vitro,with the application of cell colony formation. Methods ： Culture Human nasopharygeal carcinoma cell line CNE-2. The cytotoxicities of Cisplatin, Carboplatin, Oxaliplatin and Nedaplatin on CNE-2 were determined in96-well plates with 5×103 cells each well through WST to obtain IC10 and IC50. The IC10 of platinum compounds was used as radiation sensitizing working concentration. Collect CNE-2 cells of the log phase. After digesting, resuspending, counting, according to the different ray dose gradient in a dish,add cell suspension of corresponding total cell number to the culture dishes. Experiment was divided into normal control group, the irradiation group, alone drug group, drug + radiation group;the drug + radiation group is divided into four subgroups: cisplatin +irradiation group, carboplatin + irradiation group, oxaliplatin + irradiation group, nedaplatin + irradiationgroup. Set three Petri dish for each group. The IC10 working concentration of cisplatin, carboplatin, oxaliplatin, nedaplatin was added in 24 hours beforeirradiation with medical linear accelarator in 6MV X ray. Each group were given 0、0.5、1、2、3、4、6、8、10Gy of irradiation dose.The dose rate was 412.31cGy/min, SSD=100cm, PDD=97.232%,the irradiation area was 30×30cm. Fresh medium was replaced in four hours after irradiation, theCNE-2 cells cultured in the incubator at 37 ° C, 0.5% CO2 thermostat for 14d, records the killing effect of different treatments, calculate the survival rate, clone formation rate with application of counting colony-forming method;the mathematical model of the single-hit multi-target was used to plot survival curve (the Gr