2014年大学本科毕业的设计精选100温度盐度对不同壳色马氏珠母贝CAT酶活性联合效应的研究.docVIP

目录 摘要 I abstract II 1 前言 1 2 材料与方法 2 2.1 实验材料 2 2.2 实验设计 2 2.3 实验方法 3 2.4 蛋白含量和酶活力的测定 3 2.5 计算方法 3 2.6 数据统计与分析 3 3 实验结果 4 3.1 模型的方差分析 4 3.2 模型的显著性检验 6 3.3 响应曲面分析 8 4 讨论 13 4.1 过氧化氢酶的作用机理 13 4.2 温度、盐度单因素对四种壳色马氏珠母贝CAT活性的影响 13 4.3 温度、盐度的互作效应对四种壳色马氏珠母贝CAT活性的影响 14 鸣谢 15 参考文献 16 摘要 采用中心复合设计和响应曲面分析法研究了温度（18℃-34℃）、盐度（20-40）对四种壳色马氏珠母贝CAT）活性的联合效应。设定温度范围为18-34℃，盐度范围为20-40，对四种壳色马氏珠母贝CAT的定量模型，结果表明：温度的一次效应白、黑、红壳色马氏珠母贝CAT活性影响极显著（P0.01），对黄壳色马氏珠母贝CAT活性影响不显著（P0.05）盐度的一次效应对红、黄壳色马氏珠母贝CAT活性影响显著（P0.05），对白、黑壳色马氏珠母贝CAT活性影响不显著；温盐互作效应对白、黑、黄壳色马氏珠母贝CAT影响显著（P0.05），对红壳色马氏珠母贝CAT影响极显著（P0.01）。温度的二次效应对四种壳色马氏珠母贝CAT影响极显著（P0.01）。盐度的二次效应对四壳色马氏珠母贝CAT影响极显著（P0.01）。 白、黑红、黄四个壳色家系的回归方程的决定系数范围为0.9843-0.0.9905，校正决定系数范围为0.9687-0.9811，预测决定系数范围为0.8524-0.9190。表明所建立的四个方程拟合度高，模型恰当。 关键词：马氏珠母贝；壳色；过氧化氢酶；联合效应abstractThe combined effects of temperature and salinity on the CAT in Pinctada martensii of the four shell colors families by using of central composite design and response surface method were studied, The range of temperature is 18℃-34℃.and salinity is 20-40. This study established the equations of responses. The results showed as that: Liner effects of temperature on the CAT in white, block and red shell colors were highly significant (P0.01), not significant in yellow shell color(P 0.05); Liner effect of salinity on the CAT in red, yellow shell colors were significant (P0.05), but not significant in the white, block shell colors(P0.05); Interaction effect of temperature and salinity on the CAT in white, block and yellow shell colors were significant(P0.05), highly significant in red shell color(P0.01); Quadratic effects of temperature on the CAT in four shell colors were highly significant(P0.01); Quadratic effects of salinity on the CAT in four shell colors were highly significant(P0.01); The model equations of the CAT towards temperature and salinity ware established, with R2 being 0.9687-0.9811, the establishment of the model is appropriate . Keywords: Pinctada martensii; Shell color; CAT; Combined effec