布鲁氏菌蛋白质抗原靶向化树突状细胞疫苗研制-development of dendritic cell vaccine targeting brucella protein antigen.docxVIP

the adjuvant-MONTANIDETM ISA 50V (Seppic System), and the mice were hypodemically injected. The mice were subscribed on 4d after the first inoculation and on 3d, 5d and 7d respectively after the second booster. The serum was prepared for the analysis of IFN-γ. The results suggested that the response could not retain a long time, though the Brucella vaccine induced the higher level of IFN-γ immune response in a short time. However, the level of IFN-γ constantly increased and was notably higher than the Brucella vaccine and purified proteins from 5d to 7d after the second inoculation (P＜0.05). These results suggested that the glycosylated proteins induced the efficient Th1 immune response. Furthermore, the most important was that the glycosylated proteins elicited stronger and longer immunological memory than the brucella vaccine. Our research suggests that the 37.5 kD and 47.2 kD OMPs have good immunogenicity. The glycosylated proteins enhance the engulf capability and antigen presentation of DCs that promote the Th1 cellular immune response. Therefore, the Brucella protein antigen- targeted DCs vaccine is absolutely feasible. Key words: brucella; macrophage; dendritic cell; mannose receptor; glycosylation 缩 略 词 索 引 表 英文缩写 TNF 英文全称 Tumor necrosis factor 中文译名 肿瘤坏死因子 DCs Dendritic cell 树突状细胞 MHC Major histocompatibility complex 主要组织相容性复合体 APC Antigen presenting cell 抗原提呈细胞 CD Cluster of differentiation 白细胞分化抗原 NK Natural killer 自然杀伤 MR Mannose receptor 甘露糖受体 IL Interleukin 白细胞介素 PG Peptidoglycan 肽聚糖 IFN Interferon 干扰素 PBS Phosphate buffered solution 磷酸盐缓冲液 OMPs Outer membrane proteins 外膜蛋白 LPS Lipopolysaccacride 脂多糖 BSA Albumin bovine V 牛血清白蛋白 SDS Sodium dodecyl sulphate 十二烷基硫酸钠 OD Optical density 光密度 RB51 Rough strain B.abortus 51 粗糙型牛种布鲁氏菌 51 VTRSl Rough mutant B.suis 猪布鲁氏菌突变株 VTRM1 Rough mutant B. melitensis 16M 羊种布鲁氏菌突变株 独创性声明 本人声明所呈交的学位论文是本人在导师指导下进行的研究工作及取得的研究 成果。据本人所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已 经发表或撰写过的研究成果，也不包含为获得 河北农业大学 或其他教育机构的 学位或证书而使用过的材料。与我一同工作的同志对