磁性荧光peg-pcl聚合物胶束的制备表征及用于细胞双模式成像的初步分析-preparation and characterization of magnetic fluorescent peg - pcl polymer micelles and preliminary analysis for dual mode imaging of cells.docxVIP

华 中华 中 科 技 大 学 硕 士 学 位 论文IIIIAbstractPolymeric micelles have been emerged as an excellent nano-carrier for hydrophobic molecules or nanoparticles owing to their special properties of small and controllable size, good stability, modification ability and so on. Magnetic-fluorescent polymeric micelles which combining the advantages of polymeric micelles, have double functions of fluorescence and magnetic response and attract extensive interest of researchers owing to their great potential for biomedical applications. The objective of the project was to construct the magnetic-fluorescent polymeric micelles with cancer cell targeting ability which could be used as a multimodal imaging probe for MRI and fluorescent imaging. Based on the block copolymer PEG-PCL as a carrier, and SPIO as an MRI T2 contrast agent, the following work was carried out:Solvent-evaporation method was used to prepare SPIO-loaded mPEG5000-PCL5000 micelles. The average size of the micelles was 100 ± 15 nm and the PDI was 0.166 ±0.021 detected by a Malvern Zatasizer. Moreover, the morphology of the micelles was investigated by transmission electron microscopy (TEM). The result showed that the micelles were spherical in shape with inner core and outer shell. No obvious aggregation was observed.The T2 relaxivity of the mPEG5000-PCL5000 micelles was 86 mM-1s-1 detected byMRI system. The micelles had significant enhancing effect of MRI T2-weight image contrast. Cell uptake experiment indicated that the micelles which had some magnetic response could be internalized by Hela cells.mPEG5800-PCL15000 and NH2-PEG5800-PCL19000 were used as carrier materials for SPIO-loaded micelles preparation according to the same method mentioned before. Then Cy5.5 and transferrin were conjugated to the micelles. The average size of the modified micelles was about 255 nm and the PDI was 0.042 detected by a Malvern Zatasizer. TEM images of the micelles showed that the single micelle was isolated clusters of SPIO particles.