骨形态发生蛋白-7bmp-7对脑缺血再灌注损伤时nf-κbp65和bax表达的影响-effect of bone morphogenetic protein - 7 bmp - 7 on nf - κ bp65 and bax expression in cerebral ischemia-reperfusion injury.docxVIP

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骨形态发生蛋白-7bmp-7对脑缺血再灌注损伤时nf-κbp65和bax表达的影响-effect of bone morphogenetic protein - 7 bmp - 7 on nf - κ bp65 and bax expression in cerebral ischemia-reperfusion injury.docx

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骨形态发生蛋白-7bmp-7对脑缺血再灌注损伤时nf-κbp65和bax表达的影响-effect of bone morphogenetic protein - 7 bmp - 7 on nf - κ bp65 and bax expression in cerebral ischemia-reperfusion injury

万方数据万方数据AbstractObjective: To study the neuroprotective effects of bone morphogenetic protein-7(BMP-7) in rats models of cerebral ischemia-reperfusion injury, whether it isrelated with inhibitors of apoptosis proteins NF-κB and Bax .Methods: A total of 70 adult healthy male Wistar rats were divided into three equal groups (except 10 failure model): the sham-operating group, model group, BMP-7 treatedgroup. They were established as middle cerebral ischemia-reperfusion (I/R) injury modelby thread tying method. The rats treated with BMP-7 were immediately intervened with BMP-7(0.1 mg·kg－1) via caudal vein after I/R, meanwhile the rats in the other two groups were injected with equal volume of saline. After reperfusion 24 hours, the rats’neurological deficits scores were assessed; the apoptotic volume of crerbral were detected in TUNEL assay; the expression of apoptosis related proteins NF- κ B and Bax weredescribed by means of immunohistochemical (IHC) method.Results: There were no neurological function imperfection and infarction discovered in the sham-group. Compared with model group after I/R 24 hours, the neurological deficitsscores of rats, infarction volume and apoptotic cells in treatment group were decreasedobviously. And the differences between the two groups have statistical significance(P0.05). The results of IHC implied that the MD of proteins NF- κ B inBMP-7 treated group were higher than the model group and that of Bax were significantly lower than the model group. And it has statistical significance (P0.05).Conclusion: BMP-7 play an important role in I/R injury neurons. It provides protective effect on cerebral ischemia-reperfusion injury through promoting the activity of NF-κB which resists apoptosis and decreasing the expression of Bax which promote apoptosis.Postgraduate student: Songmei Yu(Emergency Medicine) Directed by Prof. Haitao PeiKey words: Bone Morphogenetic Proteins; ischemia reperfusion injury; NF-kappa B；Bcl-2-Associated X Prot