骨碎补总黄酮对大鼠牙髓干细胞增殖和成骨分化的作用分析-effect of total flavonoids from drynaria fortunei on proliferation and osteogenic differentiation of rat dental pulp stem cells.docxVIP

华中科技大学硕士学位论文华中科技大学硕士学位论文55of DPSCs with the total flavonoids of drynaria with the concentration of 0.01 g/L, 0.05 g/L and 0.1 g/L increased as a function of time (P0.05). The OD490 of 0.01 g/L group was significantly increased when compared with the 0.1 g/L group (P0.05), while the OD490 of0.1 g/L and 0.05 g/L groups were similar (P﹥0.05). The OD490 of all groups at 5th, 7th and9th day was higher than that of 3th day (P0.05). The ALP activities of treated groups obviously increased with the concentrations of total flavonoids of drynaria (P0.05). The volume and number of calcified nodules in treated groups was significantly increased than control group (P0.05).3. The expression level of pAkt increased with the concentrations of Drynaria total flavonoid (P0.05).Conclusions: The total flavonoids of drynaria could promote the proliferation and osteogenic differentiation of rat DPSCs and this effect might rely on the PI3K/Akt pathway. Key words: dental pulp stem cell; total flavonoids of drynaria; PI3K/Akt pathway; proliferation; osteogenic differentiation11缩略词表英文缩写英文全称中文全称BMSCsBone marrow stem cells骨髓间充质干细胞DPSCsDental pulp stem cells牙髓干细胞PI3KPhosphatidylinosito-3-kinases磷酸肌醇 3-激酶AktSerine/threonine kinase色氨酸/苏氨酸激酶pAktPhosphor-Akt磷酸化色氨酸/苏氨酸激酶mTORMammal target of rapamycin哺乳动物雷帕霉素靶蛋白GSK3Glycogen synthases kinase 3糖原合成激酶 3NF-κBNuclear factor-kappaB核结合因子 kappaBFBSFatal bovine serum胎牛血清STRO-1Stromal cell antigen-1基质细胞抗原-1MTTMetromethyl thiazolyl blue四甲基偶氮噻唑蓝ODOptical density光密度IODIntegrated optical density积分光密度ALPAlkaline phophatase碱性磷酸酶DMSODimethyl sulfoxide二甲基亚砜EDTAEthylenediamineteraacdtic acid乙二胺四乙酸DABDiaminobenzidime对氨基联苯胺PBSPhosphate-buffered saline磷酸盐缓冲液SDSSodium dodecyl sulfate十二烷基硫酸钠PAGEPolyacrylamide-gel electrophoresis聚丙烯酰胺凝胶电泳PIP2Phosphatidy linositol bisphosphate二磷酸肌醇PIP3Phosphatidy linositol trisphosphate三磷酸肌醇独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取 得的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何 其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人 和集体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本 人承担。学位论文作者签名