两起新型布尼亚病毒感染病例病毒序列分析.docVIP

两起新型布尼亚病毒感染病例病毒序列分析 　　【摘要】 目的：分析发热伴血小板减少综合征布尼亚病毒感染（SFTSV）感染患者的临床特点、流行病学以及SFTSV基因序列。方法：收集本市2014年4-6月某医院送检的2例重症发热伴血小板减少（SFTS）患者的血清标本，经核酸荧光PCR检测阳性确诊。对病毒核酸测序并与GenBank数据比对。结果：两名患者均有典型的SFTSV感染的临床症状，以发热、腹泻为首发症状，血常规见粒系及血小板呈进行性降低。从2例患者血清提取的核酸经测序，其基因序列与GenBank中的SFTSV进行比对，0428株（安庆）的RNA多聚酶、糖蛋白、NS蛋白的同源性与安徽代表株AHL/China/2011的同源性最高，均达到99%。0507株（滁州）上述目的基因与江苏株JS2011-013-1的同源性高达99%。结论：SFTSV在本地区散发存在，基因序列分析结果提示其来源或有差异。 　　【关键词】 布尼亚病毒感染； 发热； 血小板减少； 基因； 病毒； 序列分析 　　Viral Sequence Analysis of Two Cases with SFTSV Infections/SHI Li-min， HE Min， QIAO Meng-kai， et al.//Medical Innovation of China，2014，11（31）：018-020 　　【Abstract】 Objective： To investigate the clinical characteristics and epidemiology of patients infected with severe fever with thrombocytopenia syndrome bunyavirus （SFTSV） and the genetic background of the SFTSV. Method： Clinical and epidemiological data of two cases with severe fever with thrombocytopenia syndrome （SFTS）， collected from a hospital in Nanjing during April 2014 to June 2014， were retrospectively analyzed. Nucleic acid was extracted from the serum of the two SFTSV cases. After specific polymerase chain reaction（PCR）， the nucleic acid sequences were compared with those in GenBank using the BLAST facility. Result： Two patients presented typical clinical symptoms of SFTSV infection， with acute onset of fever and diarrhea. Routine blood examination showed that neutrophils and platelets progressively decreased. The results of alignment with database in GenBank， showed that RNA-dependent RNA polymerase gene， the glycoprotein gene and NS protein gene of the sample 0428（Anqing） had 99% homology with AHL/China/2011， the representative strain of AnHui Province. While the genes of sample 0507（Chuzhou） had 99% homology with JS2011-013-1， the representative strain of JiangSu Province. Conclusion： The results suggest that SFTSV occur sporadically in Nanjing and its origin might be diverse. 　　【Key words】 SFTS bunyavirus； Fever； Thrombocytopenia syndrome； Gene； Viral； Sequence ana