下调细胞色素P450表达与模拟妊娠高血压综合征实验研究.docVIP

下调细胞色素P450表达与模拟妊娠高血压综合征实验研究 　　【摘要】 目的 探讨细胞色素P450表达与妊娠高血压的关系。方法 比较下调细胞色素P450表达对妊娠晚期血压以及对胎鼠发育的影响,运用有创血压测定,胎鼠和胎盘发育指标以及western blot,评价细胞色素P450表达与胎鼠发育的关系。结果 与对照组相比,抑制细胞色素P450之后,实验组血压明显升高(118.6±8) mm Hg vs(95.1±7)mm Hg,P0.05),实验组的胎鼠重量、身长和胎盘的重量都较对照组显著降低(1.8±0.39)g vs(2.76±0.61)g,(1.78±0.32)vs(3.04±0.58)cm,(0.38±0.12)vs(0.45±0.15)g,P0.05),同时实验组胎盘中CYP2C23表达显著下降。结论 抑制胎盘CYP2C23表达可能是导致胎鼠发育受限制的原因,下调细胞色素P450表达是妊娠高血压的重要发病机制。? 　　【关键词】 妊娠高血压综合征;胎儿发育;花生四烯酸 ?? 　　Downregulation of cytochrome P450 expression to mimics Pregnancy induced hypertension 　　HUANG Hui, CHEN Jie, XIE Zhi-yong,et al. 　　The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120,China 　　【Abstract】 Objective To study the relation of cytochrome P450 expression and pregnancy induced hypertension. Methods Compared the changes after inhibition of cytochrome P450 expression, to evaluate the function of cytochrome P450 in pregnancy induced hypertension with blood pressure measure, fetus development and western blot.Results Comparing with control group, the blood pressure increase(118.6±8)vs(95.1±7)mm Hg,P0.05),the weight and length of fetus, and the weight of placenta decrease(1.8±0.39)vs(2.76±0.61)g,(1.78±0.32)vs(3.04±0.58)cm,(0.38±0.12)vs(0.45±0.15)g,P0.05),western blot showed the expression of CYP2C23 was inhibited by MSPPOH in plancta. Conclusion These results demonstrated the inhibition of CYP2C23 expression was the cause of fetus retardation. Downregulation of cytochrome P450 expression might be the mechanism of pregnancy induced hypertension.? 　　【Key words】 Pregnancy induced hypertension;Fetus development;Arachidonic acid 　　? 　　妊娠高血压综合征(Pregnancy induced hypertension,PIH,简称妊高症)为严重影响母婴安全的疾病,属于我国孕产妇主要死亡原因,也是造成胎儿宫内发育不良和早期胎儿死亡的最重要原因,其防治极为重要。然而目前众多的发病 　　DOI:10.3760/cma.j.issn 1673-8799.2010.11.16? 　　作者单位:510120中山大学附属第二医院(黄辉 陈洁 陈慧 陈仁华 王景峰); 　　中山大学附属药学院(谢智勇) 　　机制均未能较好地解释该疾病[1],我们前期的工作已经发现,在正常妊娠的生理变化过程中,肾脏微血管细胞色素P450(cytochrome P450,简称CYP)表达有周期性变化[2]。本研究在此基础上,通过N-methanesulfonyl-6-(2- proparyloxyphenyl)hex