脑缺血缺氧中缺氧诱导因子hif-1α对小胶质细胞自噬调节的机制研究-mechanism of hypoxia inducible factor hif - 1α regulating microglia autophagy during cerebral ischemia and hypoxia.docx

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脑缺血缺氧中缺氧诱导因子hif-1α对小胶质细胞自噬调节的机制研究-mechanism of hypoxia inducible factor hif - 1α regulating microglia autophagy during cerebral ischemia and hypoxia.docx

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脑缺血缺氧中缺氧诱导因子hif-1α对小胶质细胞自噬调节的机制研究-mechanism of hypoxia inducible factor hif - 1α regulating microglia autophagy during cerebral ischemia and hypoxia

缩写语表英文缩写英文全称中文对照DMEMDulbecco’smodifiedeagle’smediumDMEM培养基DMSODimethylsulphoxide二甲基亚砜DTTDithiothreitol二硫苏糖醇hhour小时IкBInhibitorofnuclearfactorkappaB核因子кB抑制因子Mgmilligram毫克Minminute分Mlmilliliter毫升3-(4,5-dimethylthyiazol-2-yl)-2,5-dipheylMTTtetrazolliumbromide四甲基偶氮唑盐IL-8Interleukin-8白细胞介素8NCNormalcontrol正常对照NF-кBNuclearfactorkappaB核因子кBODOpticaldensity光密度值OGDOxygenglucosedeprivation氧糖剥夺PBSPhosphatebufferedsaline磷酸盐缓冲液PVDFPolyvinylidenedifluoridemembrane聚二氟乙烯膜rpmrevolutionsperminute每分钟转速ELISAEnzyme-linkedimmunosorbentassay酶联免疫吸附实验SDSSodiumdodecylsulfatepolyacrylamidegel十二烷基硫酸钠聚丙烯酰胺RT-PCRReverseTranscription-PolymeraseChainReaction逆转录-聚合酶链式反应TBSTrisbufferedsaline三羟甲基氨基甲烷缓冲盐IL-1βInterleukin-1β白细胞介素1βTrisTrisaminomethane三羟甲基氨基甲烷TritonX-100TritonX-100辛基酚聚乙二醇醚Tween20Tween20吐温201RNAiRNAinterferenceRNA干扰mgmicrogram毫克GFPGreenfluorescentprotein绿色荧光蛋白mlmicroliter毫升mmolmicromole毫摩尔PCRPolymeraseChainReaction聚合酶链反应WBWesternblotting蛋白质印迹法2Themechanismstudyofhypoxiainducingfactor-1alphainthemicrogliaautophagyofcerebralischemia/hypoxiaAbstractIschemicstrokeisthediseaseofnervedysfunctionduetodisruptionofthecerebralbloodflow.Inoldpeople,thediseaseincidenceisrising,whichwilldoharmtohumanhealth,resultingindisabilityandhighfatalityrate.Accordingtothesurveyoftherelatedresearch,thenumberofischemiccerebralelderlypatientshasreachedaround1.2-1.5million,thedeathreachedto0.8-1millioninChina.Insurvivingpatients,morethanhalfofthepeoplehavevaryingdegreesofneurologicaldisability.Withtheincreasingagingofthepopulation,theischemicstrokenumberwillalsoincrease.Therefore,ischemiccerebralstrokehasbecomethecurrentmajordiseasetoourhealth.Thestudyofischemiccerebralstrokepathogenesisandtherelatedtreatmentstrategieshaveanimportantsignificanceinclinic.Thecurrentpathogenesisandtreatmentofischemiccerebralstrokehaveproposedthenewconcept-neurovascularnetwork.Thetheorysuggestthatvascularendothelialcells,nervecells,gliacellsandextracellularmatrixsurroundingcompositionsuchasorganiccomplexformedofneurovascularunit.Influenc