同种异体组织工程软骨修复猪膝关节软骨缺损可行性的研究.pdfVIP

ABSTRACT Objective: The main purpose of this study is to investigate the potential of repairing the artilcular cartilage defect by using the homologous tissue engineering cartilage formed by fetal bone marrow mesenchymal stem cells(BMSCs). Method: 1. Fetal pigs were obtained by the uterine-incision delivery of the 70 days pregnant pigs, and primary fetal BMSCs(fBMSCs) were isolated from the bone marrow. Primary adult BMSCs(aBMSCs) were isolated from the bone marrow which was aspirated from iliac. Morphology of these two kinds of cells were observed after in vitro proliferation. Then we depiceted the growth curve of two kinds of BMSCs. The BMSCs were characterized by their osteogenic, adipogenic and chonrogenic differentiation potential. The proliferation and multiple differentitation potential were evaluated to detect the biological properties of fBMSCs and aBMSCs. 2. The fBMSCs and aBMSCs were respectively seeded onto a polyglycolic acid/polylactic acid (PGA/PLA) scaffold at the concentration of 108/ml. All specimens were harvested after in vitro culture for 8 weeks. Gross observation, glycosaminoglycan(GAG) quantification, total collagen   万方数据 quantification, and histology were used to compare cartilage-related characteristic differences of engineered cartilage formed by the two kinds of cells. 3. The pig with full-thickness cartilage defects in the articular was establishes as an in vivo resesrch model. The cartilage formed by fetal BMSCs was used to repair the ca