沙葱胚胎学及种子休眠机理的分析-analysis of embryology and seed dormancy mechanism of allium mongolicum regel.docx

absorbing water, which is one of the main causes of seed dormancy. 2.2. The leach liquor of alliummongolicum seed has inhibitory effect on germination rate of cabbage seed, which not only can inhibit seed germination, effect on seedling growth, but also can cause seedling teratogenesis, etc., it illustrates that intrinsic inhibitor contained in alliummongolicum seed which can inhibit germination is one of the causes of dormancy. The embryo of alliummongolicum seed is cultured in the MS basic medium, the embryo can germinate fast, and the germination rate is up to 85%, so the embryo is not the main cause of alliummongolicum seed dormancy, and at the same time it illustrates that intrinsic inhibitor is mainly contained in the seed coat. The inhibitor of germination is contained in various organic phases of dormant alliummongolicum seed, which not only can inhibit germination rate, seedling height and root length of the cabbage seed, but also can inhibit its seed itself, the seed which dormancy is broken also contains inhibitor of germination which is less than the dormant seed, but mainly focuses on the methanol phase. GC-MS analysis shows variety of germination inhibitory material is isolated and identified from organic-phase leach liquor of the alliummongolicum seed, mainly organic acids, esters, phenols, benzenes, aldehydes, alcohols, ketones, etc. The content of endogenous ABA in the seed increases rapidly firstly and then decreases with the extension of lamination time, GA content increases rapidly firstly and then decreases, IAA becomes fluctuant, the content increases considerably one day before dormancy is broken, ZR content shows a downward trend overall. The ratio of (GA＋IAA)/ABA and IAA/ABA shows a similar variation trend, both ratio increase with the extension of lamination time, both ratio come up to the maximum when it is the 12th-day lamination, and it is the time when the seed dormancy is relieved. To sum up, alliummongolicum seed’s dormancy mechanism