神经病理痛大鼠drg神经元gaba a受体激活电流的变化-changes of gaba a receptor activation current in drg neurons of neuropathic pain rats.docxVIP

- - II - Abstract Objective: To investigate the changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain. Methods: The neuropathic pain model was established by chronic constriction injury(CCI) 7 days before electrophysiologic al recording. The rat DRG neurons were enzymatically dissociated. Whole-cell patch clamp technique was used to record GABA-activated currents. The changes of currents of injured side and opposite side were expected to compared with control group. Results: (1) The currents of injured side of CCI group were notablely decreased compared with control group(GABA concentration, 0.01-1000 μ mol/ L) (P0.01). 0.01-0.1 μ mol/ L GABA does not cause visible GABA- activated currents, 1 μ mol/ L GABA cause 0. 16 ± 0. 01 nA (n = 3) GABA-activated currents, 10 μ mol/ L GABA cause 0. 59 ± 0. 06 nA (n =7) GABA-activated currents,100 μ mol/ L GABA cause 0. 97 ± 0. 05 nA (n = 8) GABA-activated currents,1000 μ mol/ L GABA cause 1. 18 ± 0. 07 nA (n = 3) GABA-activated currents. (2) By the contrast, opposite side currents of CCI group increased significantly compared with control group(GABA concentration, 0.01-1000 μ mol/ L) (P0.01). 0.01 μ mol/ L GABA cause 0. 14 ± 0. 02 nA (n = 4) GABA-activated currents,0.1 μ mol/ L GABA cause 0. 29 ± 0. 03 nA (n = 7) GABA-activated currents,1 μ mol/ L GABA cause 1. 53 ± 0. 29 nA (n = 6) GABA-activated currents, 10 μ mol/ L GABA cause 2. 53 ± 0. 31 nA (n =6) GABA-activated currents,100 μ mol/ L GABA cause 4. 27 ± 0. 37 nA (n = 6) GABA-activated currents,1000 μ mol/ L GABA cause 4. 30 ± 0. 22 nA (n = 4) GABA-activated currents. Conclusion: The data indicates that the chronic constriction injury both change the function of GABAA recepters of injury side and opposite side. The decrease of presynaptic inhibition of GABA may be the possible reason of neuropathic pain. Key words: Neuropathic pain ; Dorsal root ganglion (DRG); GABA; Whole-cell patch clamp Type of thesis: