双眼形觉剥夺成年大鼠视皮层可塑性与cspgs和tpa表达关系的分析-relationship between plasticity of visual cortex and expressions of cs pgs and tpa in adult rats with binocular form deprivation.docxVIP

increasedincontralateralvisualcortexcausedbymonoculardeprivationincriticalperiod.WhetheritcanalsohappeninBFDisnotknown.Overall,wemadethishypothesis:tPAactivityincreasesinvisualcortexinresponsetobinocularformdeprivationinadultrats,whichdegradesCSPGs,redusesexpressionofPNNs,andthen“reactivates”adultvisualcortexplasticity.Thereforeweappliedthefollowingmethodstoinvestigatethishypothesis:1、Patternvisualevokedpotentials(PVEPs)wasappliedtodetecttheODshiftinordertofindouttheendtimeofvisualcortexplasticityandthepropertimetoreactiveadultvisualcortexplasticitybybinocularformdeprivationinadultrats.Results：(1)Brief3daysmonoculardeprivationcausedtheODshiftinPW3、PW4andPW5rats,whichshowedthevisualcortexplasticityexisted.(2)TheODshiftcouldnotbeobservedinratsafterPW6afterbrief3daysmonoculardeprevation,whichshowedthevisualcortexplasticitywasinhibited.(3)AsignificantODshiftwasobservedinPW7ratsafter14days’BFDinresponsetobrief3daysmonoculardeprivation,whichdemonstratedasteadyreactivationofvisualcortexplasticityinadultrats.(4)14days’BFDwastakenasthemodelinthefollowingexperiments.2、Withtheuseofimmunofluorescencehistochemistry,immunoblotandELISAkit,wedetectedtPAexpressionandactivityinvisualcortexduringthecriticalperiodand14days’BFDinadultrats.Results：(1)TherewereonlyafewcellsexpressingtPAinvisualcortexinPW1,andincreasedsignificantlyaftereyes’open.TheexpressionoftPAdependedonthevisualexperience.(2)Highexpressionandactivitywasdetectedinthepeakofcriticalperiod,butloweroneinadultvisualcortex.TPAplayedaroleintheendofvisualcortexplasticity.(3)TheexpressionandactivityoftPAwasincreasedinBFD,whichshowedBFDaffectedtheexpressionandactivityoftPA.3、WiththeuseofimmunofluorescencehistochemistrytodoublelabletPAandCSPGsinvisualcortexduringthecriticalperiodand14days’BFDinadultrats.Results：(1)NoCSPGswasdetectedinPW1,andsomeweredetectedaftereyes’openandthen,increasedwiththeage.TheexpressionofCSPGsdependedonthevisualexperienceandtheage,whicharerelevanttotheendofvisualcortexplasticity.(2)ExpressionofCSPGsdecreas